showForm Anti-pex-peroxysomal-marker antibody

Name:
Phone:
E-mail:
Message

Pex14p | Peroxisomal marker

AS08 372 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Magnaporthe oryzae, Zea mays

Pex14p | Peroxisomal marker in the group Antibodies for Plant/Algal  / Compartment Markers / Peroxisomal marker at Agrisera AB (Antibodies for research) (AS08 372)

DATA SHEET IN PDF

Qty: 
278
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p UniProt:Q9FE40, TAIR: At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 were replaced with a dual StrepII-His6 tag.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 25 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

55.5 | 75-65 kDa (depending on the gel system)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Solanum tuberosum, Zea mays
Predicted reactivity Cucumis melo, Magnaporthe oryzae
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot with plant peroxisomal marker antibody

Twenty 5-day-old light grown Arabidopsis thaliana seedlings (wild type, the pex14-2 null allele, and the pex14-1 truncation allele) were ground with a pestle in a 1.5 mL tube on dry ice following the addition of a double volume (60 μL) of NuPAGE 2x loading buffer (Invitrogen). After centrifugation, 20 μL of supernatant was trasnferred to a fresh tube with 2.1 μL 0.5 M DTT and boiled at 100 °C for 5 mins. Samples were loaded onto NuPAGE 10% Bis-Tris gel (Invitrogen) next to Cruz Marker (Santa Cruz Biotechnology). After electrophoresis, proteins were transferred for 30 mins at 24 V to a Hybond ECL nitrocellulose membrane (Anersham Pharmacia Biotech) using NuPAGE transfer buffer (Invitrogen). The blot was blocked for 1 h 4 °C in 8% non-fat dry milk in TBS-T (blocking buffer),and incubated overnight wiht agitation at 4 °C with primary antibody (1:10 000 rabbit anti-PEX14, AS08 372, Agrisera) diluted in blocking buffer. The antibody soliution was decanted and the blot was rinsed 3 times, 5 min each, with blocking buffer at 4 °C. The blot was incubated with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:5000), diluted in blocking buffer for 4 h at 4 °C with agitation. The blot was washed with 3 times, 5 min each, with TBS-T and developed with chemiluminescent reagent (Advanta), 1:10 dilution in water.

Courtesy Sarah Burkhart and Bonnie Bartel, Rice University, USA.

Additional information

Additional information Pex14p is prone to degradation.
Antibodies will detect Pex14 protein in both light- and dark-grown seedlings grown on petri dishes and in rosette leaves of adult plants grown in soil.

Related products

Related products

AS10 711 | Anti-DEG15 | endopeptidase, peroxisomal marker - with wider species reactivity than Arabidopsis thaliana, rabbit antibodies

AS11 1797 | Anti-HPR | hydroxypyruvate reductase (peroxysomal matrix marker), rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

Pex14p has a protein transporter activity and is involved in protein targeting into peroxisome. Submitted protein name: Genomic DNA, chromosome 5, P1 clone:MQB2

Product citations

Selected references McLoughlin et al. (2018). Maize multi-omics reveal roles for autophagic recycling in proteome remodelling and lipid turnover. Nat Plants. 2018 Dec;4(12):1056-1070. doi: 10.1038/s41477-018-0299-2.
Gonzalez et al. (2018). A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3163-E3172. doi: 10.1073/pnas.1721279115.
Vincent et al. (2017). A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749.
Zhong et al. (2016). MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae. PLoS Pathog. 2016 Aug 24;12(8):e1005823. doi: 10.1371/journal.ppat.1005823. eCollection 2016.
Li et al. (2015). A Chaperone Function of NO CATALASE ACTIVITY1 Is Required to Maintain Catalase Activity and for Multiple Stress Responses in Arabidopsis. Plant Cell. 2015 Feb 19. pii: tpc.114.135095.

Related products: Pex14p | Peroxisomal marker

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS10 711 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Physcomitrel...
340 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
190 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
185 €