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Phosphothreonine

AS18 4171   | Clonality: polyclonal  |  Host: Rabbit  |  Reactivity: phosphothreonine

Phosphothreonine in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS18 4171)

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Product Information

Immunogen KLH-conjugated phosphothreonine
Host Rabbit
Clonality Polyclonal
Purity Affinity purified
Format Liquid
Quantity 400 µl
Storage Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0.01 % sodium azide. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)

Reactivity

Confirmed reactivity Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml.

2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.

Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions. 

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Background

Background Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.

Product citations

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