Phosphothreonine

Name: Phosphothreonine
Brand: IKP
SKU: AS18 4171
Price: 377 EUR
Availability: InStock

AS18 4171 | Clonality: polyclonal | Host: Rabbit | Reactivity: phosphothreonine

Phosphothreonine in the group Antibodies Plant/Algal / Plant Developmental Biology / Plant Signal Transduction at Agrisera AB (Antibodies for research) (AS18 4171)

Product Information

Immunogen KLH-conjugated phosphothreonine

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.

Format Liquid

Quantity 400 µl

Storage Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)

Reactivity

Confirmed reactivity Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml.
This antibody is recognizing proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.

2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.

Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.

Background

Background Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.

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immunogen:	KLH-conjugated phosphothreonine
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.
Format:	Liquid
Quantity:	400 µl
storage:	Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)
recommended dilution:	ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)
Confirmed reactivity:	Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine. 5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml. This antibody is recognizing proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.
additional information (application):	2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium. Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.
background:	Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.