Phosphothreonine
AS18 4171 | Clonality: polyclonal | Host: Rabbit | Reactivity: phosphothreonine

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Product Information
Immunogen
KLH-conjugated phosphothreonine
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.
Format
Liquid
Quantity
400 ĩl
Storage
Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution
ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)
Reactivity
Confirmed reactivity
Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml.
This antibody is recognizing proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.
This antibody is recognizing proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.
2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.
Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.
Background
Background
Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.
Product citations
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