Product no: AS18 4171

AS18 4171   | Clonality: polyclonal  |  Host: Rabbit  |  Reactivity: phosphothreonine

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  • Product Info
  • Immunogen: KLH-conjugated phosphothreonine
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.
    Format: Liquid
    Quantity: 400 µl
    Storage: Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)
    Recommended dilution: ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)
  • Reactivity
  • Confirmed reactivity: Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

    5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml.
    This antibody is recognizing  proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.
    Additional information (application):

    2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.

    Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions. 

  • Background
  • Background: Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.
  • Product Citations
  • Selected references: Weise Havshøi et al. (2024). The mechanism behind tenuazonic acid-mediated inhibition of plant plasma membrane H+-ATPase and plant growth. JBC 107167.
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