AS18 4171   | Clonality: polyclonal  |  Host: Rabbit  |  Reactivity: phosphothreonine

Phosphothreonine in the group Antibodies Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS18 4171)


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen KLH-conjugated phosphothreonine
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.
Format Liquid
Quantity 400 ĩl
Storage Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)


Confirmed reactivity Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml.
This antibody is recognizing  proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.

2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.

Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions. 

Related products


Background Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.

Product citations

Related products: Phosphothreonine

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS13 2704  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:phosph...
357 €
AS13 2669 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plant...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items