AS18 4171 | Clonality: polyclonal | Host: Rabbit | Reactivity: phosphothreonine
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5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.
Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.