Phosphotyrosine (clone PY20)
AS20 4378 | Clonality: Monoclonal | Host: Mouse | Reactivity: Phosphotyrosine
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Immunogen balbC mice immunized with phosphotyrosine coupled to carrier protein
Purity Affinity purified in PBS with 0.08% sodium azide
Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution To be determined by end user
Confirmed reactivity Phosphotyrosine - not species dependent
Not reactive in No confirmed exceptions from predicted reactivity are currently known
Background Phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function.
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