PHR1 | Phosphate starvation response 1 (monocots)

Product no: AS18 4222

AS18 4222   | Clonality: Polyclonal  |  Host: Rabbit | Reactivity: Hordeum vulgare

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from  Hordeum vulgare PHR1, UniProt: F4Y5E9
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Lyophilized antibody can be stored at -20C for up to 3 years. Re-constituted antibody can be stored at 4C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 33 kDa
  • Reactivity
  • Confirmed reactivity: Hordeum vulgare (recombinant PHR1)
    Predicted reactivity: Aegilops tauschii, Brachypodium distachyon, Dichanthelium oligosanthes, Panicum hallii 
    Species of your interest not listed? Contact us
    Not reactive in: dicots
  • Application Examples
  • Application example


    Western blot using anti-Hordeum vulgare PHR1 antibodies


    Samples: purified MBP tag protein, 50 ng, (1), purified MBP-HvTF (MYB-family like PHR1) tag protein 50 ng, (2), purified MBP+HvPHR1, 50 ng, (3).  Samples were separated on 13% SDS-PAGE and blotted 1h to PVDF/nitrocellulose using semi-dry transfer. Blot was blocked with 10% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:50 000 in 10% milk with TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with chemiluminescent detection reagent. Exposure time was 3 minutes.

    Courtesy of Msc. Paweł Sega, Institute of Molecular Biology and Biotechnology, Department of Gene Expression, UAM, Poznań, Poland

  • Background
  • Background: PHR1 (Phosphate starvation response 1) is nuclear localized and involved in transcription regulation.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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