Anti-PHR1 | Phosphate starvation response 1 (monocots) antibodies

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  PHR1 | Phosphate starvation response 1 (monocots)
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      <p><strong>AS18 4222 </strong>  | Clonality: <strong>Polyclonal </strong> |  Host: <strong>Rabbit </strong>| Reactivity: <em><strong>Hordeum vulgare</strong></em></p>  
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<p><a href="/en/artiklar/phr1-phosphate-starvation-response-1-monocots.pdf?skrivpdf=j"><img src="/bilder/ibutik-a/symboler/ikon_pdf.gif" border="0" align="absmiddle" hspace="3" vspace="3"> DATA SHEET IN PDF</a></p>

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              <strong>How to cite this product:</strong><br /><span>Product name, number (Agrisera, Sweden)</span>
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      <h3>Product Information</h3>
          
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          <span>Immunogen</span>
          <span><a href="https://www.agrisera.com/shop?funk=visa_artikel&artnr=AS99%20001" target="_blank" rel="noopener noreferrer">KLH</a>-conjugated peptide derived from  <em>Hordeum vulgare</em> PHR1, UniProt: <a href="https://www.uniprot.org/uniprot/F4Y5E9" target="_blank" rel="noopener">F4Y5E9</a></span>
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          <span>Host</span>
          <span>Rabbit</span>
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          <span>Clonality</span>
          <span>Polyclonal</span>
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          <span>Purity</span>
          <span>Affinity purified serum, in PBS pH 7.4</span>
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          <span>Format</span>
          <span>Lyophilized</span>
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          <span>Quantity</span>
          <span>50 µg</span>
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          <span>Reconstitution</span>
          <span>For reconstitution add 50 µl, of sterile water.</span>
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          <span>Storage</span>
          <span>Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. <br />Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.</span>
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          <span>Tested applications</span>
          <span>Western blot (WB)</span>
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          <span>Recommended dilution</span>
          <span>1 : 1000 (WB)</span>
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          <span>Expected | apparent MW</span>
          <span>33 kDa</span>
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      <h3>Reactivity</h3>
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          <span>Confirmed reactivity</span>
          <span><em>Hordeum vulgare </em>(recombinant PHR1)</span>
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          <span>Predicted reactivity</span>
          <span><em>Aegilops tauschii, Brachypodium distachyon, Dichanthelium oligosanthes, Panicum hallii </em><br /> Species of your interest not listed?  <a href=https://www.agrisera.com/en/info/contact-us.html target=_blank rel=noopener noreferrer>Contact us</a></span>
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          <span>Not reactive in</span>
          <span>dicots</span>
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              <span>Application examples</span>
              <span><strong>Application example<br /><br /></strong> <br /><img src="https://www.agrisera.com/dokument/bibliotek/Image/AS18 4222.jpg" alt="Western blot using anti-Hordeum vulgare PHR1 antibodies" width="389" height="549" /><br /><br /><br />
<p>Samples: purified MBP tag protein, 50 ng,<strong> (1)</strong>, purified MBP-HvTF (MYB-family like PHR1) tag protein 50 ng,<strong> (2)</strong>, purified MBP+HvPHR1, 50 ng,<strong> (3)</strong>.  Samples were separated on 13% SDS-PAGE and blotted 1h to PVDF/nitrocellulose using semi-dry transfer. Blot was blocked with 10% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:50 000 in 10% milk with TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with chemiluminescent detection reagent. Exposure time was 3 minutes.<br /><br /></p>
<p>Courtesy of Msc. Paweł Sega, Institute of Molecular Biology and Biotechnology, Department of Gene Expression, UAM, Poznań, Poland</p></span>
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       <h3>Related products</h3>
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          <span>Related products</span>
          <span><a href="https://www.agrisera.com/en/artiklar/plantalgal-cell-biology/hordeum-vulgare/ALLA/sida.html" target="_blank" rel="noopener">Collection of antibodies to barley</a></span>
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     <section id="background">
       <h3>Background</h3>
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              <span>Background</span>
              <span><span id="admin_page_title"><span class="rubrik_namn_liten"><strong>PHR1 (Phosphate starvation response 1) </strong>is nuclear localized and involved in transcription regulation.<br /></span></span></span>
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<p><strong>Agrisera supports these plant societies <br /><a href="https://aspb.org/" target="_blank" rel="noopener" title="American Society of Plant Biology"><img src="https://www.agrisera.com/dokument/bibliotek/Image/aspb.png" width="41" height="40" alt="" /></a>    <a href="https://www.fespb.org/" target="_blank" rel="noopener" title="The Federation of European Society of Plant Biology"><img src="https://www.agrisera.com/dokument/bibliotek/Image/fesbp_logo_green_.png" width="39" height="40" alt="" /></a>    <a href="http://cspb-scbv.ca/" target="_blank" rel="noopener" title="Canadian Society of Plant Biologists"><img src="https://www.agrisera.com/dokument/bibliotek/Image/Canadian Society Logo 2 color_rotated_transparent_350.gif" width="40" height="40" alt="" /></a>    <a href="http://www.photosynthesisresearch.org/" target="_blank" rel="noopener" title="International Society of Photosynthesis Research"><img src="https://www.agrisera.com/dokument/bibliotek/Image/ispr_.png" width="47" height="50" alt="" /></a>    <a href="http://spps.se/" target="_blank" rel="noopener" title="Scandinavian Plant Physiology Society"><img src="https://www.agrisera.com/dokument/bibliotek/Image/SPPS_trans.png" width="52" height="37" alt="" /></a></strong><a style="padding: 4px 8px;" href="https://aspb.org/" title="American Society of Plant Biology" target="_blank" rel="noopener"></a></p>
<p><strong>The PSL Alliance</strong></p>
<p><a href="https://pivotalscientific.com/our-consultancy-services/the-psl-alliance" target="_blank" rel="noopener" title="The PSL Alliance"><img src="https://www.agrisera.com/dokument/bibliotek/Image/psl_.png" width="67" height="40" alt="" /></a></p>
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