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PHR1 | Phosphate starvation response 1 (monocots)
AS18 4222 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare
- Product Info
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Immunogen: KLH-conjugated peptide derived from Hordeum vulgare PHR1, UniProt: F4Y5E9 Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 33 kDa - Reactivity
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Confirmed reactivity: Hordeum vulgare (recombinant PHR1) Predicted reactivity: Aegilops tauschii, Brachypodium distachyon, Dichanthelium oligosanthes, Panicum hallii
Species of your interest not listed? Contact usNot reactive in: dicots - Application Examples
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Application example
Samples: purified MBP tag protein, 50 ng, (1), purified MBP-HvTF (MYB-family like PHR1) tag protein 50 ng, (2), purified MBP+HvPHR1, 50 ng, (3). Samples were separated on 13% SDS-PAGE and blotted 1h to PVDF/nitrocellulose using semi-dry transfer. Blot was blocked with 10% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:50 000 in 10% milk with TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 3 min with chemiluminescent detection reagent. Exposure time was 3 minutes.
Courtesy of Msc. Paweł Sega, Institute of Molecular Biology and Biotechnology, Department of Gene Expression, UAM, Poznań, Poland
- Background
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Background: PHR1 (Phosphate starvation response 1) is nuclear localized and involved in transcription regulation.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
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This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
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AS16 ECL-S-1L, 1L
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