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PhyB | Phytochrome B (dicots)

AS21 4566  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

PhyB | Phytochrome B (dicots) in the group Antibodies Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS21 4566)



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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PhyB, C-terminal, UniProt: P14713, TAIR: At2g18790
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 129 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabis alpina, Camelina sativa, Capsella rubella, Brassica napus,  Brassica oleracea, Eutrema salsugineum, Raphanus sativus

Species of your interest not listed? Contact us
Not reactive in Hordeum vulgare, Poplar sp., Zea mays

Application examples

Application examples Western blot using anti-PhyB polyclonal antibodies

Samples:
Ler - 30 ug of Arabidopsis thaliana Ler dark grown seedling extract
phyB-5 - 30 ug of Arabidopsis thaliana phyB-5 mutant dark grown seedling extract
MW markers: PageRuler Prestained Protein Ladder (Thermo Fisher Scientific)

30 μg/well of total protein extracted from 4-days-old Arabidopsis thaliana seedlings with extraction buffer (65 mM Tris/HCl pH 6,8; 4 M Urea; 10% glycerol; 3% SDS; 0.05% bromphenol blue; 20 mM DTT). Protein extracts were stored overnight at -20°C. Prior to separation on SDS PAGE, extracts were thawed and incubated for 5 min at 65°C followed by centrifugation and were separated on 9% SDS-PAGE and blotted 2 h to PVDF membrane (Immobilon-P pore size 0.45 μm) using wet transfer. Blot was blocked with 5% milk powder in TBS-T for 1 h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 in TBS-T ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed with TBS-T briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 5 min with AgriseraECLSuperBright with Fusion SL (Peqlab) luminescence camera system. Exposure time was 8 minutes at full resolution.

Courtesy of Dr. Andreas Hiltbrunner, Freiburg University, Germany

Additional information

Additional information PhyB | Phytochrome B (dicots)

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Background

Background PhyB (Phytochrome B) is a Red/far-red photoreceptor involved in the regulation of de-etiolation. Protein exists in two inter-convertible forms: Pr and Pfr (active). Involved in the light-promotion of seed germination and in the shade avoidance response. Alternative names: Protein LONG HYPOCOTYL 3, Protein OUT OF PHASE 1, OOP1.

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