Anti-PIN2 | Auxin efflux carrier component 2 (polyclonal)

Product no: AS20 4521

AS20 4521   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated mixture of two synthetic peptides derived from AtPIN2 sequence, UniProt:Q9LU77, TAIR:At5g57090
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL)
    Recommended dilution: 1 : 200 (IL)
    Expected | apparent MW: 69.3 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Beta vulgaris, Brassica napus, Camelina sativa, Cannabis sativa, Capsella rubella, Cucumis melo, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Malus domestica, Morus notabilis, Prunus dulcis, Raphanus sativus, Spinacia oleracea, Vitis vinifera

    Species of your interest not listed? Contact us
    Not reactive in: Lemna minor, Zea mays
  • Application Examples
  • Immunolocalization using anti-AtPIN2 polyclonal antibodies

    Left panel: epidermal layer of the root shown polar PIN2 localization. 
    Right panel: The PIN2 signals were restricted only to cortex, epidermis and root caps. 

    Material: Arabidopsist thaliana young seedling-5-day-old roots.
    Fixation: 2 % Formaldehyde in Microtuble-stabilizing buffer stock solution (50 mM PIPES, 5 mM EGTA, 2 mM MgSO4, 0.4 % Triton), vacuum for 3 min. and fixation conducted for 40 min. 
    Washing buffer: Microtuble-stabilizing buffer stock solution (50 mM PIPES, 5 mM EGTA, 2 mM MgSO4, 0.4 % Triton)
    Primary antibody: 1: 200
    Secondary antibody: anti-rabbit (1:250): (Rhodamine Red™-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (H+L)/Jackson ImmunoResearch/ Cat# 711-295-152) 

    And the unique signal pattern colocalized with PIN2-GFP marker line (in green). The PIN2 signal is not visible in the middle of a root tissue, where PIN2 protein does not occur (negative control). 

    Immunofluorescence using anti plant PIN2 rabbit polyclonal antibodies

    Material: Arabidopsist thaliana young seedling-5-day-old roots.
    Fixation: 4 % Formaldehyde in 1×Phosphate buffered saline (PBS) (4g NaCl, 0.115g NaH2PO4•H2O, 0.695g Na2HPO4•2H2O, 0.1g KCl in 1L ddH2O, pH 7.4) supplement with 0.1% TritonX, vacuum for 1 hour, and fixation conducted for 1 hour.
    Washing buffer: 1×Phosphate buffered saline (PBS)
    Blcoking buffer: 3% BSA (Carl Roth, cat. No. 8076.3) in 1×PBS
    Primary antibody: 1: 500 ON/4ºC
    Secondary antibody: anti-rabbit (1:500), Alexa Fluor 568 (Invitrogen, A11011).

    Courtesy SHEN Jinbo Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, China

  • Background
  • Background: Arabidopsis thaliana auxin efflux carrier component AtPIN2 encoded by the AtPIN2 gene (also known as EIR1 and AGR1). AtPIN proteins are asymmetrically localized within plant plasma membranes and mediate polar auxin transport. AtPIN2 is a key regulator of the response of Arabidopsis roots to gravity. Alternative names: Auxin efflux carrier AGR, Ethylene-insensitive root 1, AtEIR1, Polar-auxin-transport efflux component AGR1, Protein AGRAVITROPIC 1, AtAGR1, Protein WAVY 6.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

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AS07 260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items