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PIN2 | Auxin efflux carrier component 2

AS20 4521   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

PIN2 | Auxin efflux carrier component 2 in the group Antibodies Plant/Algal  / Hormones / Auxins at Agrisera AB (Antibodies for research) (AS20 4521)
PIN2 | Auxin efflux carrier component 2



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Product Information

Immunogen KLH-conjugated mixture of two synthetic peptides derived from AtPIN2 sequence, UniProt:Q9LU77, TAIR:At5g57090
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl, of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunolocalization (IL)
Recommended dilution 1 : 200 (IL)
Expected | apparent MW 69,3 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Beta vulgaris, Brassica napus, Camelina sativa, Cannabis sativa, Capsella rubella, Cucumis melo, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Malus domestica, Morus notabilis, Prunus dulcis, Raphanus sativus, Spinacia oleracea, Vitis vinifera

Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Immunolocalization using anti-AtPIN2 polyclonal antibodies

Left panel: epidermal layer of the root shown polar PIN2 localization. 
Right panel: The PIN2 signals were restricted only to cortex, epidermis and root caps. 

Material: Arabidopsist thaliana young seedling-5-day-old roots.
Fixation: 2 % Formaldehyde in Microtuble-stabilizing buffer stock solution (50 mM PIPES, 5 mM EGTA, 2 mM MgSO4, 0.4 % Triton), vacuum for 3 min. and fixation conducted for 40 min. 
Washing buffer: Microtuble-stabilizing buffer stock solution (50 mM PIPES, 5 mM EGTA, 2 mM MgSO4, 0.4 % Triton)
Primary antibody: 1: 200
Secondary antibody: anti-rabbit (1:250): (Rhodamine Red™-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (H+L)/Jackson ImmunoResearch/ Cat# 711-295-152) 

And the unique signal pattern colocalized with PIN2-GFP marker line (in green). The PIN2 signal is not visible in the middle of a root tissue, where PIN2 protein does not occur (negative control). 

Immunofluorescence using anti plant PIN2 rabbit polyclonal antibodies

Material: Arabidopsist thaliana young seedling-5-day-old roots.
Fixation: 4 % Formaldehyde in 1×Phosphate buffered saline (PBS) (4g NaCl, 0.115g NaH2PO4•H2O, 0.695g Na2HPO4•2H2O, 0.1g KCl in 1L ddH2O, pH 7.4) supplement with 0.1% TritonX, vacuum for 1 hour, and fixation conducted for 1 hour.
Washing buffer: 1×Phosphate buffered saline (PBS)
Blcoking buffer: 3% BSA (Carl Roth, cat. No. 8076.3) in 1×PBS
Primary antibody: 1: 500 ON/4ºC
Secondary antibody: anti-rabbit (1:500), Alexa Fluor 568 (Invitrogen, A11011).

Courtesy SHEN Jinbo Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, China

Additional information

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Background

Background Arabidopsis thaliana auxin efflux carrier component AtPIN2 encoded by the AtPIN2 gene (also known as EIR1 and AGR1). AtPIN proteins are asymmetrically localized within plant plasma membranes and mediate polar auxin transport. AtPIN2 is a key regulator of the response of Arabidopsis roots to gravity. Alternative names: Auxin efflux carrier AGR, Ethylene-insensitive root 1, AtEIR1, Polar-auxin-transport efflux component AGR1, Protein AGRAVITROPIC 1, AtAGR1, Protein WAVY 6.

Product citations

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