PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5 | Aquaporins

Product no: AS22 4816

AS22 4816   | Clonality: Polyclonal |  Host:Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide derived from N terminus of Raphanus sativus PAQ1O80368. Chosen peptide is conserved in PIP1;1, PIP1;2, PIP1;3 N-terminus of Raphanus sativus and in  all 5 isoforms of Arabidopsis thaliana coded by:AT3G61430.1 (PIP1;1), AT2G45960.3 (PIP1;2), AT1G01620.1 (PIP1;3), AT4G00430.1 (PIP1;4), AT4G23400.1 (PIP1;5)
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 30.66 | 28 kDa 
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Amaranthus tricolor, Beta vulgaris subsp. vulgaris, Lupinus sp. , Hordeum vulgare, Nicotiana tabacum, Phoenix dactilifera, Raphanus sativus, Solanu lycopersicum, Ricinus communis, Oryza sativa, Populus trichocarpa, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-PIP1 antibodies

    40-50 µg/well of total protein extracted freshly from Arabidopsis thaliana roots.  Exact buffer components were: 50mM Tris-HCl pH 8, 20mM EDTA, 1mM DTT, 1% PIC and 400mM Sucrose and denatured with 3X sample buffer (62.5mM Tris pH-6.8 , 10% glycerin, 2% SDS, 5% Mercaptoethanol, 0,05% Bromophenolblue) at 70°C/5 min.  Samples were separated in the cold on 8,5 % SDS-PAGE and blotted for 1h nitrocellulose (pore size of 0,45µm), using: wet transfer in the cold. Blot was blocked with 5 % milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 with agitation in TBS-T ON/4°C. The antibody solution was decanted, and the blot was washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in 3%Milk for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instruction. Exposure time was 1 second.

    Courtesy of Chaitra Hiremat, University of Natural Resources and Life Science, Vienna, Austria

  • Background
  • Background: PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. PIP1;2 is also designated as PIP1B.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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