Anti-PIP2;1, PIP2;2, PIP2;3 | Plasma membrane intrinistic protein 2-1, 2-2, 2-3

Product no: AS22 4810

AS22 4810 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana (recombinant target protein)

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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2 proteins: P43286, At3g53420 , AtPIP2-2 P43287, At2g37170 , AtPIP2-3 P30302, At2g37180
    Host: Rabbit


    Purity: Affinity purified serum, in PBS pH 7.4


    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 30 kDa (PIP2.1); ~35 kDa ((PIP2.2);  30 kDa (PIP2.3)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-PIP2;1., PIP2;2, PIP2;3 antibodies

    1-30 ug of Arabidopsis thaliana root microsome preparation (MP) WT1, experiment 1
    2-30 ug of Arabidopsis thaliana root microsome preparation (MP) WT1, experiment 2
    3-30 ug of Arabidopsis thaliana root microsome preparation (MP) WT1, experiment 3
    Mark: MW markers (too weak to visualize)
    5-30 ug of Arabidopsis thaliana root microsome preparation (MP) WT2, experiment 1
    6-30 ug of Arabidopsis thaliana root microsome preparation (MP) WT2, experiment 2
    7-30 ug of Arabidopsis thaliana root microsome preparation (MP) WT2, experiment 3

    30 µg/well of total protein extracted freshly from Arabidopsis thaliana roots.  Exact buffer components were: 330 mM sucrose, 100 mM KCl,1 mM EDTA, 5 mM DTT, 50 mM Tris/MES, pH 7.5 + protease inhibitor cocktail, and denatured with 70°C 10 min.  Samples were separated in the 12% SDS-PAGE and blotted for 1h to nitrocellulose, using: semi-dry transfer. Blot was blocked with 0.3% BSA for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:  10 000 with agitation in TBS-T ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera AS09 602) diluted to 1: 25 000 in  for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: ÂgriseraBright. Exposure time was 6 minutes.

    Courtesy of Tatsiana Straub, University of Hohenheim, Germany

  • Background
  • Background: PIP2;2 is a plasma membrane aquaporin.
    Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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