PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7, C-terminal
AS12 2110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: L. sativa, P. sativum, S. lycopersicum, Z. mays
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KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4, conserved also in Zea mays PIP2-1, UniProt: Q84RL7, PIP2-2, UniProt: Q9ATM8, PIP2-3 (80 % conservation) UniProt: Q9ATM7, PIP2-4 (80 % conservation) UniProt: Q9ATM6, PIP2-5 (70 % conservation) UniProt: Q9XF58, PIP2-6 (50 % conservation) UniProt: Q9ATM5
30.7 | 30 kDa (Zea mays)
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10 µg of total protein from Zea mays roots (1), Phaseolus vulgaris leaves (2) or roots (3) extracted with a mixture of 250 mM sorbitol, 50 mM Tris–HCl (pH 8), 2 mM EDTA, and protease inhibitors [1 mM phenylmethylsulfonyl Xuoride, 1 mg ml-1 each of leupeptin, aprotinin, antipain, chymostatin, and pepstatin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3.000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed four times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:30 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.
Courtesy of Dr. Ricardo Aroca, CSIC, Spain
This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.
This product can be sold containing ProClin if requested.
Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane.
Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a
Related products: PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7, C-terminal
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