PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7, C-terminal

AS12 2110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: L. sativa, P. sativum, S. lycopersicum, Z. mays

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4, conserved also in Zea mays PIP2-1, UniProt: Q84RL7, PIP2-2, UniProt: Q9ATM8, PIP2-3 (80 % conservation) UniProt: Q9ATM7, PIP2-4 (80 % conservation) UniProt: Q9ATM6, PIP2-5 (70 % conservation) UniProt: Q9XF58, PIP2-6 (50 % conservation) UniProt: Q9ATM5

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 600 (IP), 1: 3000 (WB)

Expected | apparent MW

30.7 | 30 kDa (Zea mays)

Reactivity

Confirmed reactivity Lactuca sativa, Pisum sativum, Solanum lycopersicum, Zea mays

Predicted reactivity Arabidopsis thaliana, Artemisia annua, Brassica oleracea, Capsicum annuum, Capsicum chinense, Cicer arietinum, Coffea arabica, Cucumis melo, Cucumis sativus, Fragaria chiloensis, Glycine max, Helianthus annuus, Hordeum vulgare, Malus prunifolia, Medicago trunculata, Mimosa pudica, Nicotiana tabacum, Noccaea caerulescens, Olea europaea, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Prunus mume, Pyrus communis, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Trifolium repens, Triticum urartu, Triticum aestivum, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in Hordeum vulgare

Application examples

Application example

10 µg of total protein from Zea mays roots (1), Phaseolus vulgaris leaves (2) or roots (3) extracted with a mixture of 250 mM sorbitol, 50 mM Tris–HCl (pH 8), 2 mM EDTA, and protease inhibitors [1 mM phenylmethylsulfonyl Xuoride, 1 mg ml^-1 each of leupeptin, aprotinin, antipain, chymostatin, and pepstatin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3.000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed four times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:30 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesy of Dr. Ricardo Aroca, CSIC, Spain

Additional information

Detection pattern consists of di and monomer of PIP2-7.

This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.

This product can be sold containing ProClin if requested.

Related products

AS09 488 | Anti-PIP2;1 | aquaporin PIP2;1, rabbit antibodies

AS09 490 | Anti-PIP2;2 | plasma membrane aquaporin 2b, rabbit antibodies

AS09 491 | Anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodies

Plant protein extraction buffer

Background

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane.

Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a