PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7, C-terminal
AS12 2110 | Clonality: Polyclonal | Host: Rabbit | Reactivity: L. sativa, P. sativum, S. lycopersicum, Z. mays

Data sheet | Product citations | Protocols | Add review |
Product Information
KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4, conserved also in Zea mays PIP2-1, UniProt: Q84RL7, PIP2-2, UniProt: Q9ATM8, PIP2-3 (80 % conservation) UniProt: Q9ATM7, PIP2-4 (80 % conservation) UniProt: Q9ATM6, PIP2-5 (70 % conservation) UniProt: Q9XF58, PIP2-6 (50 % conservation) UniProt: Q9ATM5
30.7 | 30 kDa (Zea mays)
Reactivity
Species of your interest not listed? Contact us
Application examples
Application example
10 µg of total protein from Zea mays roots (1), Phaseolus vulgaris leaves (2) or roots (3) extracted with a mixture of 250 mM sorbitol, 50 mM Tris–HCl (pH 8), 2 mM EDTA, and protease inhibitors [1 mM phenylmethylsulfonyl Xuoride, 1 mg ml-1 each of leupeptin, aprotinin, antipain, chymostatin, and pepstatin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3.000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed four times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:30 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.
Courtesy of Dr. Ricardo Aroca, CSIC, Spain
Additional information
This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.
This product can be sold containing ProClin if requested.
Background
Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane.
Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a
Product citations
Related products: PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7, C-terminal
This product can b...