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DRP5B | Dynamin related protein 5B

AS12 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

DRP5B | Dynamin related protein 5B  in the group Plant/Algal Antibodies / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2634)

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product information
Background

DRP5B (dynamin related protein 5B), EC=3.6.5.5 is involved in plastid and peroxisome division process and required for the last steps of plastid division, especially in mesophyll-cell. Necessary for peroxisome activities. Alternative names: Dynamin-like protein ARC5, Dynamin-related protein 5B, Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DRP5B sequence UniProt: Q84N64, TAIR: AT3G19720

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

87.17 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycne max, Manihot esculenta, Mesostigma viride, Oryza sativa, Physcomitrella patens, Sorghum bicolor, Zea mays, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Most sensitive ECL reagents are necessary to obtain a reaction with this antibody.
Selected references

to be added when available, antibody released in May 2014.


application example

western blot using anti-DRP5B antibodies

Total proteins extracted from 5 mg leaf or flower bud of Arabidopsis thaliana tissue with SDS-loading buffer were separated on 10% SDS-PAGE using tank transfer and blotted for 1 h to nitrocellulose membrane. Blots were blocked with 2% non-fat dry milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (affinity-purified DRP5B) at a dilution of 1:5 000 in TBS-T + 2% non-fat dry milk overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in TBS-T + 2% non-fat dry milk for 2 hr at RT with agitation. The blot was washed as above and developed for 5 min with SuperSignal West Pico (Thermo Scientific) according to the manufacturer's instructions. Exposure time was 30 min. Additionally the same blot was washed and developed with SuperSignal West Femto (Thermo Scientific) for 1 s. Exposure time was 1 min. The latter helped to increase DRP5B detection with the affinity-purified DRP5B antibodies. The signal was not obtained from bud tissue, suggesting that expression level of DRP5B may be too low for detection (blot not included).

Courtesy of Deena Kadirjan-Kalbach and Katherine W. Osteryoung, Michigan State University, USA

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