DRP5B | Dynamin related protein 5B
AS12 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Triticum aestivum
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DRP5B sequence UniProt: Q84N64, TAIR: AT3G19720
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (WB)
Expected | apparent MW
87,17 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Triticum aestivum
Predicted reactivity
Glycne max, Manihot esculenta, Mesostigma viride, Oryza sativa, Physcomitrium patens, Sorghum bicolor, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Chlamydomonas reinhardtii
Application examples
Application examples
Application example
Total proteins extracted from 5 mg leaf or flower bud of Arabidopsis thaliana tissue with SDS-loading buffer were separated on 10% SDS-PAGE using tank transfer and blotted for 1 h to nitrocellulose membrane. Blots were blocked with 2% non-fat dry milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (affinity-purified DRP5B) at a dilution of 1:5 000 in TBS-T + 2% non-fat dry milk overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in TBS-T + 2% non-fat dry milk for 2 hr at RT with agitation. The blot was washed as above and developed for 5 min with SuperSignal West Pico (Thermo Scientific) according to the manufacturer's instructions. Exposure time was 30 min. Additionally the same blot was washed and developed with extreme low femtogram detection range chemiluminescent reagent. Exposure time was 1 min. The latter helped to increase DRP5B detection with the affinity-purified DRP5B antibodies. The signal was not obtained from bud tissue, suggesting that expression level of DRP5B may be too low for detection (blot not included).
Courtesy of Deena Kadirjan-Kalbach and Katherine W. Osteryoung, Michigan State University, USA
Total proteins extracted from 5 mg leaf or flower bud of Arabidopsis thaliana tissue with SDS-loading buffer were separated on 10% SDS-PAGE using tank transfer and blotted for 1 h to nitrocellulose membrane. Blots were blocked with 2% non-fat dry milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (affinity-purified DRP5B) at a dilution of 1:5 000 in TBS-T + 2% non-fat dry milk overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in TBS-T + 2% non-fat dry milk for 2 hr at RT with agitation. The blot was washed as above and developed for 5 min with SuperSignal West Pico (Thermo Scientific) according to the manufacturer's instructions. Exposure time was 30 min. Additionally the same blot was washed and developed with extreme low femtogram detection range chemiluminescent reagent. Exposure time was 1 min. The latter helped to increase DRP5B detection with the affinity-purified DRP5B antibodies. The signal was not obtained from bud tissue, suggesting that expression level of DRP5B may be too low for detection (blot not included).
Courtesy of Deena Kadirjan-Kalbach and Katherine W. Osteryoung, Michigan State University, USA
Additional information
Chemiluminescent extreme low femtogram detection range reagent is recommended to use with this antibody.
Background
Background
DRP5B (dynamin related protein 5B), EC=3.6.5.5 is involved in plastid and peroxisome division process and required for the last steps of plastid division, especially in mesophyll-cell. Necessary for peroxisome activities. Alternative names: Dynamin-like protein ARC5, Dynamin-related protein 5B, Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5.
Product citations
Selected references
Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.
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