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DRP5B | Dynamin related protein 5B

AS12 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

DRP5B | Dynamin related protein 5B  in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2634)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DRP5B sequence UniProt: Q84N64, TAIR: AT3G19720

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

87.17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycne max, Manihot esculenta, Mesostigma viride, Oryza sativa, Physcomitrella patens, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in Chlamydomonas reinhardtii 

Application examples

Application examples Application example

western blot using anti-DRP5B antibodies

Total proteins extracted from 5 mg leaf or flower bud of Arabidopsis thaliana tissue with SDS-loading buffer were separated on 10% SDS-PAGE using tank transfer and blotted for 1 h to nitrocellulose membrane. Blots were blocked with 2% non-fat dry milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (affinity-purified DRP5B) at a dilution of 1:5 000 in TBS-T + 2% non-fat dry milk overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:5 000 in TBS-T + 2% non-fat dry milk for 2 hr at RT with agitation. The blot was washed as above and developed for 5 min with SuperSignal West Pico (Thermo Scientific) according to the manufacturer's instructions. Exposure time was 30 min. Additionally the same blot was washed and developed with extreme low femtogram detection range chemiluminescent reagent. Exposure time was 1 min. The latter helped to increase DRP5B detection with the affinity-purified DRP5B antibodies. The signal was not obtained from bud tissue, suggesting that expression level of DRP5B may be too low for detection (blot not included).

Courtesy of Deena Kadirjan-Kalbach and Katherine W. Osteryoung, Michigan State University, USA

Additional information

Chemiluminescent extreme low femtogram detection range reagent is recommended to use with this antibody. 

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Background

Background

DRP5B (dynamin related protein 5B), EC=3.6.5.5 is involved in plastid and peroxisome division process and required for the last steps of plastid division, especially in mesophyll-cell. Necessary for peroxisome activities. Alternative names: Dynamin-like protein ARC5, Dynamin-related protein 5B, Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5.

Product citations

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