PTOX | Plastid terminal oxidase
|Recommended dilution||1 : 4000 (WB)|
|Expected | apparent MW||
30 | 37-41 kDa (Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum, Spinacia oleracea
|Predicted reactivity||Higher plants|
|Not reactive in||There is not known exception at this moment|
In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended.
|Selected references||Cournac et al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54
Josse et al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36
Cournac et al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62.
15 µg from total protein leaf extract of Spinacia oleracea (1), Arabidopsis thliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5), Arabidopsis thaliana thylakoid fraction (6) were separated on SDS-PAGE and blotted to PVDF membrane. Incubation with primary antibody: 1: 4000 followed by chemiluminescent detection. Exposure time 2 minutes.
Cross reactivity to 52 kDa band can be eliminated by using thylakoid fraction or blocking with 10 % milk ON/4°C.
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