PTOX | Plastid terminal oxidase

Name: PTOX | Plastid terminal oxidase
SKU: AS16 3692
Price: 373 EUR
Availability: InStock
Rating: 5 (2 reviews)

AS16 3692 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Araidopsis thaliana, Chlamydomonas reinhardtii

PTOX | Plastid terminal oxidase in the group Antibodies Plant/Algal / Photosynthesis / Electron transfer at Agrisera AB (Antibodies for research) (AS16 3692)

Product Information

Immunogen

Part of Arabidopsis thaliana recombinant plastid terminal oxidase, TAIR: At4g22260, UniProt: Q56X52

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 4000 (WB)

Expected | apparent MW

30 | 37-41 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum, Spinacia oleracea

Predicted reactivity Higher plants
Species of your interest not listed? Contact us

Not reactive in Phaeodactylum tricornutum

Application examples

Application examples Application example

15 µg from total protein leaf extract of Spinacia oleracea (1), Arabidopsis thliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5), Arabidopsis thaliana thylakoid fraction (6) were separated on SDS-PAGE and blotted to PVDF membrane. Incubation with primary antibody: 1: 4000 followed by chemiluminescent detection. Exposure time 2 minutes.

Cross reactivity to 52 kDa band can be eliminated by using thylakoid fraction or blocking with 10 % milk ON/4°C.

Additional information

Additional information This product can be sold containing proclin if requested

In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended

Background

PTOX (plastid terminal oxidase) is a component of electron transfer chain responsible for desaturation of phytoene, which prevents the generation of reactive oxygen species. It is involved in the differentiation of plastids: chloroplasts, amyloplasts, and etioplasts. PTOX is expressed ubiquitously in plant tissues and is located in the lumen.

Synonymes: IM, IM1, immutants, AOX4, alternative oxidase 4, ubiquinol oxidase 4, chloroplastic/chromoplastic

Product citations

Selected references Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105024
Pralon et al. (2020). Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in Arabidopsis thaliana. Front. Plant Sci., 25 March 2020
Bolte et al. (2020). Dynamics of the localization of the plastid terminal oxidase PTOX inside the chloroplast. J Exp Bot. 2020 Feb 15. pii: eraa074. doi: 10.1093/jxb/eraa074.
Cournac et al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54
Cournac et al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62.
Josse et al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36

immunogen:	Part of Arabidopsis thaliana recombinant plastid terminal oxidase, TAIR: At4g22260, UniProt: Q56X52
Reconstitution:	For reconstitution add 100 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 4000 (WB)
calculated \| apparent molecular mass [kDa]:	30 \| 37-41 kDa (Arabidopsis thaliana)
Confirmed reactivity:	Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum, Spinacia oleracea
predicted reactivity:	Higher plants Species of your interest not listed? Contact us
not reactive in:	Phaeodactylum tricornutum
Picture (footer):	Application example 15 µg from total protein leaf extract of Spinacia oleracea (1), Arabidopsis thliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5), Arabidopsis thaliana thylakoid fraction (6) were separated on SDS-PAGE and blotted to PVDF membrane. Incubation with primary antibody: 1: 4000 followed by chemiluminescent detection. Exposure time 2 minutes. Cross reactivity to 52 kDa band can be eliminated by using thylakoid fraction or blocking with 10 % milk ON/4°C.
additional information:	This product can be sold containing proclin if requested
additional information (application):	In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended
background:	PTOX (plastid terminal oxidase) is a component of electron transfer chain responsible for desaturation of phytoene, which prevents the generation of reactive oxygen species. It is involved in the differentiation of plastids: chloroplasts, amyloplasts, and etioplasts. PTOX is expressed ubiquitously in plant tissues and is located in the lumen. Synonymes: IM, IM1, immutants, AOX4, alternative oxidase 4, ubiquinol oxidase 4, chloroplastic/chromoplastic
All references:	Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105024 Pralon et al. (2020). Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in Arabidopsis thaliana. Front. Plant Sci., 25 March 2020 Bolte et al. (2020). Dynamics of the localization of the plastid terminal oxidase PTOX inside the chloroplast. J Exp Bot. 2020 Feb 15. pii: eraa074. doi: 10.1093/jxb/eraa074. Cournac et al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54 Cournac et al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62. Josse et al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36