PsbC | CP43 protein of PSII

AS11 1787 |  Clonality: Polyclonal  |  Host: Rabbit | Reactivity: A. thaliana, , C. reinhardtii, Echinochloa crus-galli, H. vulgare, O. sativa, P.sativum, P. vulgaris, Synechococcus sp., T. aestivum, Z. mays, V. lychnitis, V. radiate

PsbC | CP43 protein of PSII in the group Plant/Algal Antibodies / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS11 1787)


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product information

PsbC (CP43) acts as an antenna to the PSII core and its presence seem to be also necessary for maintaining water splitting activity. This protein is more weakly associated with the PSII reaction centre and can be removed from the isolated core.


KLH-conjugated synthetic peptide chosen from known sequences of PsbC including  Arabidopsis thaliana PsbC, UniProt: P56778, TAIR: AtCg00280

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS10 111S | CP43' | IsiA homolog of plant CP43 protein standard PSII antibody collection

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This product can be sold containing proclin if requested.

application information
Recommended dilution 1 : 3 000 (WB)
Expected | apparent MW

45 | 43 kDa

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Echinochloa crus-galli, Hordeum vulgare, Oryza sativa, Panax ginseng, Pisum sativum, Phaseolus vulgaris, Synechocystis sp. PCC6803, Triticum aestivum, Zea mays, Verbascum lychnitis, Vigna radiate
Predicted reactivity

Asimina parviflora, Borago officinalis, Cannabis sativa, Carthamus persicus, Casimirella guaranitica , Catalpa bungei, Calatola mollis, Citron x limon, Cunninghamia lanceolata, Deeringothamnus rugelii, Gonystylus bancanus, Ipomopsis aggregata, Leretia cordata, Lobatiriccardia lobata, Myricaria germanica , Nostoc sp. PCC7120, Nannochloropsis sp., Natsiatum herpeticum, Nothapodytes montana , Nerium oleander, Ottoschulzia rhodoxylon, Oxandra lanceolata,Solanum tuberosum, Oryza sativa, Panax quinquefolius, Prosopidastrum angusticarpum, Prosopis glandulosa, Rollinia mucosa, Rosmarinus officinalis, Saxifraga rivularis, Spinacia oleracea, Zelkova serrata, Zinnia violacea, Vachellia caven, Vitis vinifera, Xerocladia viridiramis

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information This product can be sold containing ProClin if requested

In C4 plants like Echinochloa crus-galli and Zea mays antibody detects 2 bands.
Selected references Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.
Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.
Chen et al. (2016). Expression of holo-proteorhodopsin in Synechocystis sp. PCC 6803. Metab Eng. 2016 Feb 8;35:83-94. doi: 10.1016/j.ymben.2016.02.001.
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Calderon et al. (2013). A Conserved Rubredoxin is Necessary for Photosystem II Accumulation in Diverse Oxygenic Photoautotrophs. J Biol Chem. July 30. (reference for reactivity in Chlamydomonas reinhardtii)
Sakuraba et al. (2013). The green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J.
Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.

Application example

western blot detection of PsbC in several species

5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB, (3) Chlamydomonas reinhardtii total cell extracted with PEB, (4) Synechococcus sp. 7942 total cell extracted with PEB,  extracted with PEB were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 75 seconds.

western blot using anti-CP43 antibodies

1.5 µg of chlorophyll from thylakoids of various treatments of Echinochloa crus-galli (1-2), Zea mays (3-5), Pisum sativum (6-7), extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl2 and 2 mM EDTA. Samples were denatured with Laemmli buffer at 75 0C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% fatty acid free milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 overnight at 40C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera ) diluted to 1:25 000 in  1 % milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 240 seconds.

Courtesy of Dr. Wiola Wasilewska, Warsaw University, Poland

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