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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

PsbS | 22 kDa Lhc-like PSII protein (chicken)

AS03 032  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: A. thaliana, A. sativa, B. napus, H. vulgare, O. sativa, P. sylvestris, P. tremula, S. oleracea

PsbS | 22 kDa Lhc-like PSII protein (chicken) in the group Plant/Algal Antibodies / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS03 032)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence.

Immunogen

KLH-conjugated synthetic peptide derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575). This sequence is even conserved in conifers.

Host Chicken
Clonality Polyclonal
Clone
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 100 µl
Reconstitution
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS09 533 | anti-PsbS rabbit antibody

PSII  available antibodies against Photosystem II proteins

Additional information
application information
Recommended dilution 1 : 2000-1 : 4000 (WB)
Expected | apparent MW

28 | 22 kDa for Arabidopsis thaliana

Confirmed reactivity Arabidopsis thaliana, Avena sativa, Brassica napus, Hordeum vulgare, Oryza sativa, Pinus sylvestris, Populus tremula, Spinacia oleracea
Predicted reactivity Dicots, Conifers
Not reactive in

Chlamydomonas reinhardtii, Chlorella sp.

Additional information
Selected references Hubbart et al. (2012). The photoprotective protein PsbS exerts control over CO2 assimilation rate in fluctuating light in rice. The Plant J. March 2012.

application example

15 µg of Arabidopsis thaliana thylakoids from (1) PsbS-overexpressing line, (2) PsbS-deficient npq4-line, and (3) wt (col) together with (4) total leaf protein from Arabidopsis thaliana wt extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbS (AS03 032, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder.  Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Technical note(s): (a) This IgY shows reactivity to both markers loaded in lane M (MagicMark and NovexSharp, both Invitrogen), for comparison a marker lane from same filter (probed seperately with a IgG-antiserum) is shown to the right.  (b) the IgY reacts with 2 bands in leafs and thylakoids that both are absent in the PsbS-deficient npq4 line and therefore both might represent PsbS-forms with a difference in molecular weight of ~1 kDa.

 

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com