PsbS | 22 kDa Lhc-like PSII protein (chicken)

Product no: AS03 032

AS03 032  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: A. thaliana, A. sativa, B. napus, H. vulgare, O. sativa, P. sylvestris, P. tremula, S. oleracea

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575). This sequence is even conserved in conifers.

    Host: Chicken
    Clonality: Polyclonal
    Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000-1 : 4000 (WB)
    Expected | apparent MW:

    28 | 22 kDa for Arabidopsis thaliana

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Avena sativa, Brassica napus, Hordeum vulgare, Oryza sativa, Pinus sylvestris, Populus tremula, Spinacia oleracea
    Predicted reactivity: Dicots, Conifers
    Not reactive in:

    Chlamydomonas reinhardtii, Chlorella sp.

  • Application Examples
  • Application example

    15 µg of Arabidopsis thaliana thylakoids from (1) PsbS-overexpressing line, (2) PsbS-deficient npq4-line, and (3) wt (col) together with (4) total leaf protein from Arabidopsis thaliana wt extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbS (AS03 032, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder.  Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected chemiluminescent reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity). Technical note(s): (a) This IgY shows reactivity to both markers loaded in lane M (MagicMark and NovexSharp, both Invitrogen), for comparison a marker lane from same filter (probed seperately with a IgG-antiserum) is shown to the right.  (b) the IgY reacts with 2 bands in leafs and thylakoids that both are absent in the PsbS-deficient npq4 line and therefore both might represent PsbS-forms with a difference in molecular weight of ~1 kDa.

    Note: For this antibody, blocking needs to be increased to 10 % non-fat milk 1h/RT incubation.

  • Background
  • Background:

    The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence.

  • Product Citations
  • Selected references: Hubbart et al. (2012). The photoprotective protein PsbS exerts control over CO2 assimilation rate in fluctuating light in rice. The Plant J. March 2012.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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Accessories

AS09 533 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, B. corticulans, B. distachyon, C. cantabricus (Wilk.) Rchb. F, F.a bidentis, H. pilosella L., H. vulgare, L. hispanica, M. esculenta, N. tabacum, O. sativa, P. abies, P. glauca, P. strobus, R.communis, S.oleracea, S. muralis (Hedw.) Raab, T. aestivum, Z.mays

319 €
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