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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

PsbX | small subunit X of PSII

AS05 066  |  Clonality: Polyclonal  |  Host: Rabbit  | Reactivity:Arabidopsis thaliana

PsbX | small subunit X of PSII in the group Plant/Algal Antibodies / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS05 066)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

Plant PsbX is a nucelar encoded small 4 kDa protein associated with Photosystem II and found in all classes of oxygenic organisms.

Immunogen

KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence At2g06520

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

PSII available antibodies against Photosystem II proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

PsbX is a small (4 kDa) and very hydrophobic subunit of PSII. Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII. The use of 15% SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody.

application information
Recommended dilution 1 : 2500, 1 µg of chlorophyll/lane (WB)
Expected | apparent MW

4 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Dictos, Oryza sativa, Zea mays
Not reactive in

Cyanobacteria

Additional information

For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).

Selected references Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com