Plastid acyl-ACP desaturase

AS13 2668 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

Product Information

Immunogen

putative mature form of Chlamydomonas reinhardtii plastid acyl-ACP desaturase expressed in E.coli, UniProt: A8IQB8

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000-1 : 1500 (WB)

Expected | apparent MW

40 | 35 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Volvox carteri
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

20 µg of soluble protein from Chlamydomonas reinhardii, were separated on 10 % SDS-PAGE and blotted for 1h to nitrocellulose. Blots were blocked with 1 % milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the plastid acyl-ACP desaturase antibody in 1 % milk in PBS-T at a dilution 1: 1500 for 2h at RT with agitation (left panel) or pre-immune serum (right panel). The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG, ALP conjugated) diluted to 1:3 000 in for 1h at RT with agitation. The blot was washed as above and reaction was visualized using BCIP/NBT.

Courtesy Dr. Dudley Page, UCLA, USA

Additional information

Antibody dilution is going to be higher with ECL

Background

Background Plastid acyl-ACP desaturase, coded by FAB2 gene is involved in fatty acid metabolism in Chlamydomonas reinhardtii. It displays acyl-[acyl-carrier-protein] desaturase activity.