PRO1 | Profilin-1 (clone mAbPRF1a 2-14D9)

AS16 3210 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana

Product Information

Immunogen Profilin 1 (PRF1) from Arabidopsis thaliana

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2a

Purity Total IgG purified on a protein G column

Format Liquid in 0.1M PBS, pH 7.4, with 0.15M Sodium Chloride, and 0.05% Sodium Azide

Quantity 100 µg

Storage Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Immunohistochemistry (IHC), Western blot (WB)

Expected | apparent MW

14 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Exact working dilution needs to be determined by end user.

This antibody reacts with PRF1.

Related products

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin also interacts with membrane polyphospho-inositides, the Arp 2/3 complex, short proline rich stretches, and other ligands.

Product citations

Selected references Kandasamy, M.K., et al. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52(1):22-32.
McKinney, E.C., et al. (2001) Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13(5): 1179-91.