PRP39a | pre-mRNA-processing factor 39
AS14 2784 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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91 | 100 kDa
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40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL of high sensitivity, according to manufacturer's instructions. Exposure time was 1 min.
Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland
PRP39a (pre-mRNA-processing factor 39) is involved in RNA processing, mRNA 5'-splice site recognition, regulation of timing of transition from vegetative to reproductive phase.