PRP39a | pre-mRNA-processing factor 39

Product no: AS14 2784

AS14 2784 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    Recombinant, full length PRP39a of Arabidopsis thaliana , UniProt: F4I448, TAIR: At1g04080

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000-1 : 5 000 (WB)
    Expected | apparent MW:

    91 | 100 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica rapa
    Species of your interest not listed? Contact us
    Not reactive in:

     

  • Application Examples
  • application example

    western blot using antiPRP39a antibodies


    40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL of high sensitivity, according to manufacturer's instructions. Exposure time was 1 min.

    Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland
  • Background
  • Background:

    PRP39a (pre-mRNA-processing factor 39) is involved in RNA processing, mRNA 5'-splice site recognition, regulation of timing of transition from vegetative to reproductive phase.

  • Product Citations
  • Selected references: Chang et al. (2021) The U1 snRNP component RBP45d regulates temperature-responsive flowering in Arabidopsis. Plant Cell. 2021 Nov 13:koab273. doi: 10.1093/plcell/koab273. Epub ahead of print. PMID: 34791475.
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Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info

AS14 2785 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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