PRP39a | pre-mRNA-processing factor 39

Name: PRP39a | pre-mRNA-processing factor 39
SKU: AS14 2784
Price: 302 EUR
Availability: InStock

AS14 2784 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PRP39a | pre-mRNA-processing factor 39 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / RNA metabolism at Agrisera AB (Antibodies for research) (AS14 2784)

Product Information

Immunogen

Recombinant, full length PRP39a of Arabidopsis thaliana , UniProt: F4I448, TAIR: At1g04080

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000-1 : 5 000 (WB)

Expected | apparent MW

91 | 100 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica rapa
Species of your interest not listed? Contact us

Not reactive in

Application examples

Application examples application example

40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL of high sensitivity, according to manufacturer's instructions. Exposure time was 1 min.

Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland

Additional information

Background

PRP39a (pre-mRNA-processing factor 39) is involved in RNA processing, mRNA 5'-splice site recognition, regulation of timing of transition from vegetative to reproductive phase.

Product citations

Selected references Chang et al. (2021) The U1 snRNP component RBP45d regulates temperature-responsive flowering in Arabidopsis. Plant Cell. 2021 Nov 13:koab273. doi: 10.1093/plcell/koab273. Epub ahead of print. PMID: 34791475.

immunogen:	Recombinant, full length PRP39a of Arabidopsis thaliana , UniProt: F4I448, TAIR: At1g04080
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
recommended dilution:	1 : 2000-1 : 5 000 (WB)
Expected \| apparent MW:	91 \| 100 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Brassica rapa Species of your interest not listed? Contact us
not reactive in:
Picture (footer):	application example 40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL of high sensitivity, according to manufacturer's instructions. Exposure time was 1 min. Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland
background:	PRP39a (pre-mRNA-processing factor 39) is involved in RNA processing, mRNA 5'-splice site recognition, regulation of timing of transition from vegetative to reproductive phase.
All references:	Chang et al. (2021) The U1 snRNP component RBP45d regulates temperature-responsive flowering in Arabidopsis. Plant Cell. 2021 Nov 13:koab273. doi: 10.1093/plcell/koab273. Epub ahead of print. PMID: 34791475.

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