PRP39a | pre-mRNA-processing factor 39
AS14 2784 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Add review |
Product Information
Immunogen
Recombinant, full length PRP39a of Arabidopsis thaliana , UniProt: F4I448, TAIR: At1g04080
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000-1 : 5 000 (WB)
Expected | apparent MW
91 | 100 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brassica rapa
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Application examples
Application examples
application example

40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL of high sensitivity, according to manufacturer's instructions. Exposure time was 1 min.
Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland

40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL of high sensitivity, according to manufacturer's instructions. Exposure time was 1 min.
Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland
Additional information
Background
Background
PRP39a (pre-mRNA-processing factor 39) is involved in RNA processing, mRNA 5'-splice site recognition, regulation of timing of transition from vegetative to reproductive phase.
Product citations
Selected references
Chang et al. (2021) The U1 snRNP component RBP45d regulates temperature-responsive flowering in Arabidopsis. Plant Cell. 2021 Nov 13:koab273. doi: 10.1093/plcell/koab273. Epub ahead of print. PMID: 34791475.
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