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PRP40B | pre-mRNA-processing protein 40B

AS14 2785 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PRP40B | pre-mRNA-processing protein 40B in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / RNA metabolism at Agrisera AB (Antibodies for research) (AS14 2785)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Recombinant Arabidopsis thaliana PRP40b, UniProt:F4JCC1, TAIR: At3g19670

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

113 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa
Species of your interest not listed? Contact us
Not reactive in

 

Application examples

Application examples Application example


Western blot using anti/PRP40b antibodies


Total protein from rosette leaves, 30 ug of Arabidopsis thaliana whole leaf extract (prp40ab) (1), 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (2), extracted with buffer (100mM Tris, 10% glicerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL of high sensitivity. Exposure time was 60 seconds.
MW markers: PageRuler Plus Prestained (ThermoFisher)

Courtesy of Agata Stępień, Adam Mickiewicz University, Poland

Additional information

Related products

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AS14 2784 | Anti-PRP39a | pre-mRNA-processing factor 39, rabbit antibodies

collection of antibodies to micro RNA

Recommended secondary antibody for ECL detection

Plant protein extraction buffer

Background

Background

 PRP40B (pre-mRNA-processing protein 40B) protein that binds the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II and functions as a scaffold for RNA processing machineries. Ubiquitously expressed and localized to the nucleus.

Product citations

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