PRP40B | pre-mRNA-processing protein 40B
AS14 2785 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
113 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brassica rapa
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Application examples
Application examples
Application example
Total protein from rosette leaves, 30 ug of Arabidopsis thaliana whole leaf extract (prp40ab) (1), 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (2), extracted with buffer (100mM Tris, 10% glicerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL of high sensitivity. Exposure time was 60 seconds.
MW markers: PageRuler Plus Prestained (ThermoFisher)
Courtesy of Agata Stępień, Adam Mickiewicz University, Poland
Total protein from rosette leaves, 30 ug of Arabidopsis thaliana whole leaf extract (prp40ab) (1), 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (2), extracted with buffer (100mM Tris, 10% glicerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL of high sensitivity. Exposure time was 60 seconds.
MW markers: PageRuler Plus Prestained (ThermoFisher)
Courtesy of Agata Stępień, Adam Mickiewicz University, Poland
Additional information
Background
Background
PRP40B (pre-mRNA-processing protein 40B) protein that binds the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II and functions as a scaffold for RNA processing machineries. Ubiquitously expressed and localized to the nucleus.
Product citations
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