PrxQ | Peroxiredoxin, thioredoxin reductase

AS05 093 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, M. esculenta, S. oleracea, Z. mays

PrxQ | Peroxiredoxin, thioredoxin reductase

Product Information

Immunogen

His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Manihot esculenta, Spinacia oleracea, Zea mays

Predicted reactivity Marchantia polymorpha, Populus sp. , Triticum aestivum, Oryza sativa
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Manihot esculenta (Cassava)

Application: Western Blotting

Pudmed ID: 24727655

Journal: PLoS One

Figure Number: 5C

Published Date: 2014-04-15

First Author: An, F., Fan, J., et al.

Impact Factor: 2.942

Open Publication

Western blotting of Rubisco, APX and PrxQ.The expression of Rubisco, APX and PrxQ in leaves of cassava NZ199 diploid (a) and autotetraploid (b) genotypes were detected by western blotting using antiRubisco-polyclonal antibody (AS07218), anti-APX antibody (AS08368) and anti-PrxQ antibody (AS05093) from Agrisera, respectively.

Reactant: Manihot esculenta (Cassava)

Application: Western Blotting

Pudmed ID: 27881899

Journal: Plant Mol Biol Report

Figure Number: 8B

Published Date: 2016-11-25

First Author: An, F., Li, G., et al.

Impact Factor: 1.526

Open Publication

Western blotting of Rubisco and peroxiredoxin. The expressions of Rubisco (a) and peroxiredoxin (b) in apical expanded leaves of SC8and Col1046 were detected by Western blotting using anti-Rubisco polyclonal antibody (AS07218) and anti-peroxiredoxin antibody (AS05093) from Agrisera, respectively. Mr, protein marker; lines a and b, the expression of Rubisco and peroxiredoxin of SC8 in 5 °C for 0 and 10 days, respectively; lines c and d, the expression of Rubisco and peroxiredoxin of Co11046 in 5 °C for 0 and 10 days, respectively

Additional information

Additional information This product can be sold containing proclin if requested

In stroma fractions a weak background reaction at 28 kDa is visible, No crossreactivity in any thylakoid fractions

Background

Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.

Product citations

Selected references Yoshida et al. (2018). Thioredoxin-like2/2-Cys peroxiredoxin redox cascade supports oxidative thiol modulation in chloroplasts. Proc Natl Acad Sci U S A. 2018 Aug 13. pii: 201808284. doi: 10.1073/pnas.1808284115.
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Yoshida et al. (2015). Thioredoxin Selectivity for Thiol-Based Redox Regulation of Target Proteins in Chloroplasts. J Biol Chem. 2015 Apr 15. pii: jbc.M115.647545.
Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.
Wu et al. (2013). Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus. PLoS One. July 23;8(7).

Confirmed reactivity:	Arabidopsis thaliana, Manihot esculenta, Spinacia oleracea, Zea mays
predicted reactivity:	Marchantia polymorpha, Populus sp. , Triticum aestivum, Oryza sativa Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.
additional information:	This product can be sold containing proclin if requested
additional information (application):	In stroma fractions a weak background reaction at 28 kDa is visible, No crossreactivity in any thylakoid fractions
All references:	Yoshida et al. (2018). Thioredoxin-like2/2-Cys peroxiredoxin redox cascade supports oxidative thiol modulation in chloroplasts. Proc Natl Acad Sci U S A. 2018 Aug 13. pii: 201808284. doi: 10.1073/pnas.1808284115. Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22. Yoshida et al. (2015). Thioredoxin Selectivity for Thiol-Based Redox Regulation of Target Proteins in Chloroplasts. J Biol Chem. 2015 Apr 15. pii: jbc.M115.647545. Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014. Wu et al. (2013). Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus. PLoS One. July 23;8(7).
More images:	Reactant: Manihot esculenta (Cassava) Application: Western Blotting Pudmed ID: 24727655 Journal: PLoS One Figure Number: 5C Published Date: 2014-04-15 First Author: An, F., Fan, J., et al. Impact Factor: 2.942 Open Publication Western blotting of Rubisco, APX and PrxQ.The expression of Rubisco, APX and PrxQ in leaves of cassava NZ199 diploid (a) and autotetraploid (b) genotypes were detected by western blotting using antiRubisco-polyclonal antibody (AS07218), anti-APX antibody (AS08368) and anti-PrxQ antibody (AS05093) from Agrisera, respectively. Reactant: Manihot esculenta (Cassava) Application: Western Blotting Pudmed ID: 27881899 Journal: Plant Mol Biol Report Figure Number: 8B Published Date: 2016-11-25 First Author: An, F., Li, G., et al. Impact Factor: 1.526 Open Publication Western blotting of Rubisco and peroxiredoxin. The expressions of Rubisco (a) and peroxiredoxin (b) in apical expanded leaves of SC8and Col1046 were detected by Western blotting using anti-Rubisco polyclonal antibody (AS07218) and anti-peroxiredoxin antibody (AS05093) from Agrisera, respectively. Mr, protein marker; lines a and b, the expression of Rubisco and peroxiredoxin of SC8 in 5 °C for 0 and 10 days, respectively; lines c and d, the expression of Rubisco and peroxiredoxin of Co11046 in 5 °C for 0 and 10 days, respectively
calculated \| apparent molecular mass [kDa]:	16 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
Purity:	Serum
Quantity:	200 µl
recommended dilution:	1 : 5000 (WB)
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)

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