PrxQ | Peroxiredoxin, thioredoxin reductase
AS05 093 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, M. esculenta, S. oleracea, Z. mays

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Product Information
His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
16 kDa
Reactivity
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Application examples

Reactant: Manihot esculenta (Cassava)
Application: Western Blotting
Pudmed ID: 24727655
Journal: PLoS One
Figure Number: 5C
Published Date: 2014-04-15
First Author: An, F., Fan, J., et al.
Impact Factor: 2.942
Open PublicationWestern blotting of Rubisco, APX and PrxQ.The expression of Rubisco, APX and PrxQ in leaves of cassava NZ199 diploid (a) and autotetraploid (b) genotypes were detected by western blotting using antiRubisco-polyclonal antibody (AS07218), anti-APX antibody (AS08368) and anti-PrxQ antibody (AS05093) from Agrisera, respectively.

Reactant: Manihot esculenta (Cassava)
Application: Western Blotting
Pudmed ID: 27881899
Journal: Plant Mol Biol Report
Figure Number: 8B
Published Date: 2016-11-25
First Author: An, F., Li, G., et al.
Impact Factor: 1.526
Open PublicationWestern blotting of Rubisco and peroxiredoxin. The expressions of Rubisco (a) and peroxiredoxin (b) in apical expanded leaves of SC8and Col1046 were detected by Western blotting using anti-Rubisco polyclonal antibody (AS07218) and anti-peroxiredoxin antibody (AS05093) from Agrisera, respectively. Mr, protein marker; lines a and b, the expression of Rubisco and peroxiredoxin of SC8 in 5 °C for 0 and 10 days, respectively; lines c and d, the expression of Rubisco and peroxiredoxin of Co11046 in 5 °C for 0 and 10 days, respectively
Additional information
Background
Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.
Product citations
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Yoshida et al. (2015). Thioredoxin Selectivity for Thiol-Based Redox Regulation of Target Proteins in Chloroplasts. J Biol Chem. 2015 Apr 15. pii: jbc.M115.647545.
Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.
Wu et al. (2013). Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus. PLoS One. July 23;8(7).
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