PsbO | Photosystem II manganese-stabilizing polypeptide (algal)

Product no: AS21 4689

AS21 4689  | Clonality:  Polyclonal  |  Host:  Rabbit  | Reactivity: Chlamydomonas reinhardtii, Synechococcus elongatus, Synechocystis sp. 

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Chlamydomonas reinhardtii PsbO protein sequence, UniProt: P12853
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 29.9-30.5 kDa | kDa (due to N-terminal or C-terminal processing)
  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Halomicronema hongdechloris, Synechocystissp., Synechococcus elongatus

    Species of your interest not listed? Contact us
    Not reactive in: Acaryochloris marina
  • Application Examples
  • Western blot using anti-algal PsbO antibodies

    10 µg/well of total protein extracted from Chlamydomonas reinhardtii, total cell extract were denatured with Invitrogen LDS sample buffer (4X) at 70°C/5 min. and separated on Invitrogen NuPage Bis-Tris 4-12% SDS-PAGE 45 min and blotted for 1 h to Invitrogen PVDF (pore size of 0.45 µm), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1h/RT with agitation, and incubated in the primary antibody at a dilution of 1: 1000 for 1h/RT with TBS-T Blocking. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 10 min and 2 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG ALP conjugated, AS09 607 lot 2105 ) diluted to 1: 5 000 in TBS-T Blocking for 0,5h/RT with agitation. The blot was washed as above and developed with Agrisera AS19 BCIP-NBT reagent lot 03068231 for 3 minutes.
  • Background
  • Background: The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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