PsbY | Small subunit Y of PSII

AS06 114 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PsbY sequence, UniProt:O49347, TAIR: At1g67740

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 4,7 and 4,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Glycne soja , Medicago truncatula, Morus notabilis, Oryza sativa, Populus trichocarpa, Ricinus communi, Solanum chacoense, Spinacia oleracea, Theobroma cacao , Zea mays, Zostera marina
Species of your interest not listed? Contact us

Not reactive in

Cyanobacteria

Application examples

Application examples Application example

1 µg of total protein from Arabidopsis thaliana PSII enriched membranes in BBY storage buffer (20 mM MES-NaOH pH 6.3, 400 mM sorbitol, 5 mM MgCl2, 10 mM CaCl2, and 15 mM NaCl) denatured with Laemmli sample buffer at 70C for 5 min were separated on 16,5 % Tris-Tricine, 6 M Urea SDS-PAGE with a 10 % spacer gel, and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 2 % low fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above, the last wash in distilled water, and developed for 2 min with chemiluminescent detection reagent. Exposure time was 60 seconds.

Courtesy of Lotta von Sydow, KBC, Umeå, Sweden

Additional information

Background

Background PsbY (Small subunit Y of PSII) is a manganese-binding polypeptide with L-arginine metabolizing enzyme activity. It is a component of the core of photosystem II. Alternative names: psbY-A1, L-AME, L-arginine.metabolizing enzyme.

Product citations

Selected references Von Sydow et al. (2016). The PsbY protein of Arabidopsis Photosystem II is important for the redox control of cytochrome b559. Biochim Biophys Acta. 2016 May 21. pii: S0005-2728(16)30536-9. doi: 10.1016/j.bbabio.2016.05.004.

background:	PsbY (Small subunit Y of PSII) is a manganese-binding polypeptide with L-arginine metabolizing enzyme activity. It is a component of the core of photosystem II. Alternative names: psbY-A1, L-AME, L-arginine.metabolizing enzyme.
Picture (footer):	Application example 1 µg of total protein from Arabidopsis thaliana PSII enriched membranes in BBY storage buffer (20 mM MES-NaOH pH 6.3, 400 mM sorbitol, 5 mM MgCl2, 10 mM CaCl2, and 15 mM NaCl) denatured with Laemmli sample buffer at 70C for 5 min were separated on 16,5 % Tris-Tricine, 6 M Urea SDS-PAGE with a 10 % spacer gel, and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 2 % low fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above, the last wash in distilled water, and developed for 2 min with chemiluminescent detection reagent. Exposure time was 60 seconds. Courtesy of Lotta von Sydow, KBC, Umeå, Sweden
All references:	Von Sydow et al. (2016). The PsbY protein of Arabidopsis Photosystem II is important for the redox control of cytochrome b559. Biochim Biophys Acta. 2016 May 21. pii: S0005-2728(16)30536-9. doi: 10.1016/j.bbabio.2016.05.004.
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Glycne soja , Medicago truncatula, Morus notabilis, Oryza sativa, Populus trichocarpa, Ricinus communi, Solanum chacoense, Spinacia oleracea, Theobroma cacao , Zea mays, Zostera marina Species of your interest not listed? Contact us
not reactive in:	Cyanobacteria
calculated \| apparent molecular mass [kDa]:	4,7 and 4,9 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana PsbY sequence, UniProt:O49347, TAIR: At1g67740
Purity:	Serum
Quantity:	200 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)

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