PsbW | Small subunit W of PSII

Product no: AS05 060

AS05 060  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from PsbW protein sequence of Arabidopsis thaliana Uniprot: Q39194, TAIR: At2g30570

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 15 000 (WB)
    Expected | apparent MW: 13,6 | 6,1 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Spinacia oleracea
    Predicted reactivity: Oryza sativa, Physcomitrium patens, Zea mays, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in:

    Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

  • Application Examples
  • Application example

    western blot detection using PsbW antibody

    2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).  Exposure time was 1 second.

  • Background
  • Background:

    PsbW is a nuclear-encoded protein located in the thylakoid membrane of the chloroplast. It is a core component of Photosystem II. Altrnative name: PSII 6.1 kDa protein

  • Product Citations
  • Selected references: Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

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