Name:
Phone:
E-mail:
Message
Tag Antibody Set 

PsbW | Small subunit W of PSII

AS05 060  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea

PsbW | Small subunit W of PSII in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS05 060)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from PsbW protein sequence of Arabidopsis thaliana Uniprot: Q39194, TAIR: At2g30570

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 15 000 (WB)
Expected | apparent MW

13.6 | 6.1 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Spinacia oleracea
Predicted reactivity Oryza sativa, Physcomitrella patens, Zea mays, Vitis vinifera
Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Application examples

Application examples

Application example

western blot detection using PsbW antibody

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).  Exposure time was 1 second.

Additional information

Related products

Background

Background

PsbW is a nuclear-encoded protein located in the thylakoid membrane of the chloroplast. It is a core component of Photosystem II. Altrnative name: PSII 6.1 kDa protein

Product citations

Selected references Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.

Related products: PsbW | Small subunit W of PSII

AS16 ECL-SN-10 | low pico to mid femtogram and extreme low femtogram detection
15 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
186 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
181 €
AS05 059 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, P. sativum,...
355 €