PsbR | 10 kDa protein of PSII

Product no: AS05 059

AS05 059 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, P. sativum, S. oleracea, T. aestivum

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsbR UniProt: P27202, TAIR: At1g79040

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 15 000 (WB)
    Expected | apparent MW:

    14 | 10 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Colobanthus quitensis, Deschampsia antarctica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Triticum aestivum Nicotiana tabacum, Quillaja saponaria
    Predicted reactivity: Brassica campestris, Catalpa bungei, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera, Zea mays
    Species of your interest not listed? Contact us
    Not reactive in:

    Chlamydomonas reinhardtii, Hordeum vulgare, Synechococcus sp. PCC 7942

  • Application Examples
  • Application example

    western blot detection using anti-PsbR antibody

    2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 1 second.

  • Additional Information
  • Additional information:

    This product can be sold containing proclin if requested

  • Background
  • Background:

    PsbR protein is found in plant Photosystem II and anticipate to play a role in water oxidation, yet the physiological significance of PsbR has remained obscure.

  • Product Citations
  • Selected references: Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
    Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.
    Albanese et al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.
    Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
    Ido et al. (2014). Cross-Linking Evidence for Multiple Interactions of the PsbP and PsbQ Proteins in a Higher Plant Photosystem II Supercomplex. J Biol Chem. 2014 Jul 18;289(29):20150-7. doi: 0.1074/jbc.M114.574822. Epub 2014 Jun 9.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    Stefano Caffarri | 2016-09-22
    0 | 0
    Very sensitive antibody, very little cross reaction. See also Crepin et al. JBC 2016

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