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PsbR | 10 kDa protein of PSII

AS05 059 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, P. sativum, S. oleracea, T. aestivum

PsbR | 10 kDa protein of PSII in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS05 059)

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Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsbR UniProt: P27202, TAIR: At1g79040

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 15 000 (WB)
Expected | apparent MW

14 | 10 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Colobanthus quitensis, Deschampsia antarctica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Triticum aestivum Nicotiana tabacum, Quillaja saponaria
Predicted reactivity Brassica campestris, Catalpa bungei, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera, Zea mays
Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii, Hordeum vulgare, Synechococcus sp. PCC 7942

Application examples

Application examples

Application example

western blot detection using anti-PsbR antibody

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 1 second.

Additional information

Additional information

This product can be sold containing proclin if requested

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Background

Background

PsbR protein is found in plant Photosystem II and anticipate to play a role in water oxidation, yet the physiological significance of PsbR has remained obscure.

Product citations

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