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Rabbit anti-Goat IgG Fc, HRP conjugated, min. cross-reactivity to human serum

AS10 1106   | Clonality: Polyclonal  |  Host:Rabbit   |  Reactivity:Goat IgG Fc

Rabbit anti-Goat IgG Fc, HRP conjugated, min. cross-reactivity to human serum in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-goat  / HRP (horseradish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1106)

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product information
Background Rabbit anti-goat IgG Fc,HRP conjugated, is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase goat IgG.
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other HRP conjugated secondary antibodies to goat IgG
Additional information

This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or immunoglobulin light chains based on immunoelectrophoresis.

Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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