Rabbit anti-Goat IgG (H&L), HRP conjugated - trial sample

Product no: AS09 605-trial
AS09 605-trial | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Goat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

21
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: purified goat IgG, whole molecule
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified rabbit IgG.
    Format: Liquid
    Quantity: 10 l
    Storage: Store lyophilized material at 2-8C. For storage at -20C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000 - 50 000 (WB)
  • Reactivity
  • Confirmed reactivity: Goat IgG heavy and light chains (H&L)
    Predicted reactivity: Goat IgG Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using secondary antibody rabbit anti-goat immunoglobulins, HRP conjugated

    5 µg of total extract from Arabidopsis thaliana leaf (S) extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary anti-BiP antibody (AS09 615) at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, AGRISERA, AS09 602) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent in extreme low femtogram range, according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

  • Additional Information
  • Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free0,1 % (v/v) of Kathon CG is used as preservative
    Additional information (application): No reactivity is observed to non-immunoglobulin goat  serum proteins based in immunoelectrophoresis.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
  • Background
  • Background: Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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Accessories

AS10 1112  | Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: Goat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

233 €
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Info
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AS16 ECL-N |  low pico to mid femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

AS16 ECL-N-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 12 €
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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 16 €
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