Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample
AS09 627-trial | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
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Product Information
Immunogen
Purified Mouse IgG, whole molecule
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified rabbit IgG.
Format
Liquid
Quantity
10 ĩl (0,91 mg/ml)
Storage
Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
Reactivity
Confirmed reactivity
Mouse IgG heavy and light chains (H&L)
Predicted reactivity
Mouse IgG Heavy and Light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
Background
Background
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Product citations
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