showForm Rabbit anti-Mouse IgG (H&L), HRP conjugated antibody,trial sample

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Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample

AS09 627-trial | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)

Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample in the group Secondary Antibodies / Anti-Mouse / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS09 627-trial)

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Product Information

Immunogen

Purified Mouse IgG, whole molecule

Host Rabbit
Clonality Polyclonal
Purity Purified rabbit IgG
Format Liquid
Quantity 10 µl (0.91 mg/ml)
Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)

Reactivity

Confirmed reactivity Mouse IgG heavy and light chains (H&L)
Predicted reactivity Mouse IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis

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Background

Background

Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.

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to be added when available