Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample
AS09 627-trial | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
DATA SHEET IN PDF| Datasheet | Product citations | Add review |
Product Information
Immunogen
Purified Mouse IgG, whole molecule
Host
Rabbit
Clonality
Polyclonal
Purity
Purified rabbit IgG
Format
Liquid
Quantity
10 µl (0.91 mg/ml)
Storage
Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
Reactivity
Confirmed reactivity
Mouse IgG heavy and light chains (H&L)
Predicted reactivity
Mouse IgG Heavy and Light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
Background
Background
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Product citations
Selected references
to be added when available
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
