Rabbit anti-Rat IgG (H&L), FITC conjugated

Product no: AS10 1523
AS10 1523 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rat IgG (H&L)
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  • Product Info
  • Immunogen: Purified rat IgG (H&L)
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified rabbit IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Store at 2-8C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
    Recommended dilution: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
  • Additional Information
  • Additional information:

    Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

    Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins

  • Background
  • Background: Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to FITC, which binds to anti-rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
  • Product Citations
  • Selected references: Kolbert et al. (2018). Nitro-oxidative stress correlates with Se tolerance of Astragalus species. Plant Cell Physiol. 2018 May 25. doi: 10.1093/pcp/pcy099.
  • Protocols
  • Antibody protocols
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