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Rabbit anti-Rat IgG (H&L), Biotin conjugated, min. cross-reactivity to human serum

AS10 1529 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rat IgG (H&L)
Rabbit anti-Rat IgG (H&L), Biotin conjugated, min. cross-reactivity to human serum in the group Secondary Antibodies / Anti-Rat / Biotinylated at Agrisera AB (Antibodies for research) (AS10 1529)

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Product Information

Immunogen Purified rat IgG (H&L)
Host Rabbit
Clonality Polyclonal
Purity Affinity purified rabbit IgG
Format Liquid
Quantity 1 mg
Storage Store at 2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)

Reactivity

Application examples

Additional information

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins

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Background

Background Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).

Product citations

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