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RAD23c | Ubiquitin receptor RAD23c

AS19 4271  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

RAD23c | Ubiquitin receptor RAD23c in the group Antibodies for Plant/Algal  / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4271)

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Product Information

Immunogen Full length, His-tagged recombinant RAD23c of Arabidopsis thaliana protein sequence, UniProt:Q84L31-1, TAIR: At3g02540, overexpressed in E.coli
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 44.2 | ca. 61 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Antibodies recognize recombinant RAD23b and RAD23c proteins equally well but do not recognize DSK2a/b orDDI1 based on immunoblot analyses of corresponding mutants  Farmer et al. (2010).

Related products

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AS19 4258 | PAC1 | 20S Proteasome alpha subunit C1
AS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodies
AS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodies

AS19 4262 | Anti-RPT2a | Regulatory particle triple-A ATPase subunit 2a, rabbit antibodies
AS19 4263 | Anti-RPT4a | 26S proteasome AAA-ATPase subunit RPT4a, rabbit antibodies

AS19 4264  | Anti-26S proteasome regulatory subunit RPN1a , rabbit antibodies
AS19 4265 | Anti-RPN5a | 26S proteasome regulatory subunit, putative (RPN5), rabbit antibodies

AS19 4266 | Anti-RPN10 | 26S proteasome regulatory subunit RPN10 , rabbit antibodies
AS19 4267 |  Anti-RPN11 | 26S proteasome regulatory subunit RPN11, rabbit antibodies
AS19 4268 | Anti-RPN12a | 26S proteasome regulatory subunit RPN12a, rabbit antibodies

AS19 4269 | Anti-PA200 | Proteasome activator PA200, rabbit antibodies
AS19 4270 | Anti-RAD23b | Rad23 UV excision repair protein family, rabbit antibodies

collection of antibodies to protein modifications and enzymes involved in this process

Background

Background RAD23c (Ubiquitin receptor RAD23c) binds and presumably selects ubiquitin-conjugates for destruction. Prefers multiubiquitin chains rather than single ubiquitins, with a binding affinity for 'Lys-48'-linked ubiquitin chains. Acts as a ubiquitin receptor that associates with the 26S proteasomal docking subunit RPN10 for the indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP). RAD23 proteins play an essential role in the cell cycle, morphology, and fertility of plants through their delivery of UPS (ubiquitin/26S proteasome system) substrates to the 26S proteasome.

Alternative names: AtRAD23c, Putative DNA repair protein RAD23-3, RAD23-like protein 3, AtRAD23-3.

Product citations

Selected references Farmer et al. (2010). The RAD23 family provides an essential connection between the 26S proteasome and ubiquitylated proteins in Arabidopsis. Plant Cell. 2010 Jan;22(1):124-42. doi: 10.1105/tpc.109.072660.

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