RBR1 | Retinoblastoma related protein
AS11 1627 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabiodpis thaliana, Medicago sativa
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Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 31666236
Figure Number: 1C
Published Date: 2019-11-26
First Author: Leviczky, T., Molnár, E., et al.
Impact Factor: 5.272Open Publication
The expression profiles of E2FA and E2FB are distinct in the developing siliques, but overlap in the proliferation phase. (A) qRT-PCR analyses of the G2- and M-phase-specific CDKB1;1 and the seed maturation LEC2 and WRI1 genes in the developing siliques of the wild-type (WT) at four silique developmental stages (S1-S4, pictured in Fig. S1). (B) The transcript levels of the three E2Fs, namely E2FA, E2FB and E2FC, and the single RBR genes were also analysed in these silique samples by qRT-PCR. Values represent fold-changes normalised to the value of the S1 silique stage (set arbitrarily at 1). Data are meanąs.d., n=3 biological repeats. *P?0.05, **P?0.01, ***P?0.001, ****P?0.0001 (two-tailed, paired t-test between consecutive silique stages). ns, non-significant. (C) To follow the accumulation levels of RBR, E2FA, E2FB and DPB proteins in developing siliques (S1-S4) specific antibodies were used in immunoblot assays as indicated. The Ponceau-stained proteins were used as loading control. Arrowheads indicate the corresponding E2FA and DPB proteins; arrow marks a slower migrating form of DPB in S4 silique stage. Molecular weights of the specific proteins are shown on the left.
This antibody is not suitable for immunolocalization.
Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane).
For immunoprecipitation start with 2 µl and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 µg of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.
As control pre-serum for IP this product can be used, total, pre-immune IgY.
The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR
Horvath et al. (2017). Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. EMBO J. 2017 May 2;36(9):1261-1278. doi: 10.15252/embj.201694561. Epub 2017 Mar 20.
Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7.
Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.
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