RFA | Baker's yeast replication factor A
AS07 214 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae
|Recommended dilution||ChIP, 1 : 20 000 (WB)|
|Expected | apparent MW||
70 + 30 + 14 kDa
|Confirmed reactivity||Saccharomyces cerevisiae|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Antibody was also successfully used in ChIP application Holstein et al. (2014).
|Selected references||Deshpande et al. (2017). Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage. Mol Cell. 2017 Oct 19;68(2):431-445.e5. doi: 10.1016/j.molcel.2017.09.019.
Chen et al. (2017). Dihydrocoumarin, an HDAC Inhibitor, Increases DNA Damage Sensitivity by Inhibiting Rad52. Int J Mol Sci. 2017 Dec 7;18(12). pii: E2655. doi: 10.3390/ijms18122655.
Yeeles et al. (2015). Regulated eukaryotic DNA replication origin firing with purified proteins. Nature. 2015 Mar 4. doi: 10.1038/nature14285.
Holstein et al. (2014). Interplay between Nonsense-Mediated mRNA Decay and DNA Damage Response Pathways Reveals that Stn1 and Ten1 Are the Key CST Telomere-Cap Components. Cell Rep. 2014 May 22;7(4):1259-69. doi: 10.1016/j.celrep.2014.04.017. Epub 2014 May 15. (ChIP)
Deng et al. (2014). RPA antagonizes microhomology-mediated repair of DNA double-strand breaks. Nat Struct Mol Biol. 2014 Mar 9. doi: 10.1038/nsmb.2786. (western blot)
Bentensen et al. (2013). MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin contex. Nucleic Acids Res. 2013 Mar 1;41(5):3173-89. doi: 10.1093/nar/gkt051. (ChIP)
TCA precipitated protein extracts from a wild type yeast strain (S. cerevisiae) were separated on 10% gel and transferred to a PVDF membrane. Antibody was used in different dilutions: 1: 5000 (1); 1: 10 000 (2); 1: 20 000 (3);
Besides the bands for RFA1 and RFA2 an unspecific band was detected at ~150 kDa.
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