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RH3 | RNA helicase (chloroplastic)

AS13 2714 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, Z. mays

RH3 | RNA helicase (chloroplastic) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS13 2714)

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays RH3A (GRMZM2G415491_P01) and RH3B (GRMZM2G163072_P01) and Arabidopsis thaliana At5g26742

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

75 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity

Panicum italicum, Oryza brachyantha, Oryza sativa


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example


 

5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. THe blot was incubated in the primary antibody at a dilution of 1: 1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG conjugated to horse radish peroxidase) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above, and imaged by ECL using a LiCor digital imager with a 1 minute exposure.

Courtesy of Dr. Alice Barkan, University of Oregon, USA

Additional information

Related products

Background

Background

RH3 (RNA helicase) is involved in RNA synthesis, modification, cleavage and degradation. RH3 is the most abundant plastid DEAD box RNA helicase in maize and Arabidopsis thaliana.

Product citations

Selected references Bach-Pages et al. (2020). Discovering the RNA-Binding Proteome of Plant Leaves With an Improved RNA Interactome Capture Method. Biomolecules. 2020 Apr 24;10(4):E661. doi: 10.3390/biom10040661.
Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10.

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