RH3 | RNA helicase, chloroplastic

AS13 2714 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays

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product information
Background		RH3 (RNA helicase) is involved in RNA synthesis, modification, cleavage and degradation. RH3 is the most abundant plastid DEAD box RNA helicase in maize and Arabidopsis thaliana.
Immunogen		recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays RH3A (GRMZM2G415491_P01) and RH3B (GRMZM2G163072_P01)
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Immunoprecipitation (IP), Western blot (WB)
Related products		antibodies to RNA metabolism Plant and algal protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected \| apparent MW		kDa
Confirmed reactivity		Arabidopsis thaliana, Zea mays
Predicted reactivity		Panicum italicum, Oryza brachyantha, Oryza sativa
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10.

Application example

5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. THe blot was incubated in the primary antibody at a dilution of 1: 1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (BioRad goat anti-rabbit IgG conjugated to horse radish peroxidase) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above, and imaged by ECL using a LiCor digital imager with a 1 minute exposure.

Courtesy of Dr. Alice Barkan, University of Oregon, USA

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