RH3 | RNA helicase (chloroplastic)
AS13 2714 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays
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recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays RH3A (GRMZM2G415491_P01) and RH3B (GRMZM2G163072_P01) and Arabidopsis thaliana At5g26742
Panicum italicum, Oryza brachyantha, Oryza sativa
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5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. THe blot was incubated in the primary antibody at a dilution of 1: 1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG conjugated to horse radish peroxidase) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above, and imaged by ECL using a LiCor digital imager with a 1 minute exposure.
Courtesy of Dr. Alice Barkan, University of Oregon, USA
RH3 (RNA helicase) is involved in RNA synthesis, modification, cleavage and degradation. RH3 is the most abundant plastid DEAD box RNA helicase in maize and Arabidopsis thaliana.
Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10.
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