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RHD3 | Protein Root Hair Defective 3 (N-terminal)

AS20 4417 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
RHD3 | Protein Root Hair Defective 3 (N-terminal) in the group Antibodies for Plant/Algal  / Cell Wall at Agrisera AB (Antibodies for research) (AS20 4417)

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Product Information

Immunogen BSA-conjugated peptide, derived from N-terminus of Arabidopsis thaliana RHD3, UniProt: P93042, TAIR: At3g13870
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Format Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Quantity 200 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1:100 -1: 200 (WB)
Expected | apparent MW 89 | 90 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Apostasia shenzhenica, Artemisia annua, Brassica rapa, Capsicum annuum, Dichanthelium oligosanthes, Hibiscus syriacus, Mucuna pruriens, Panicum hallii, Phoenix dactylifera, Tanacetum cinerariifolium, Triticum urartu, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-RHD3 antibodies

Arabidopsis thaliana 7 days-old seedlings of wilde-type (1), rhd3-1 mutant (2), rhd3-2 mutant (3) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 15-20 % SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 100 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.

Aterisks indicate non-specific bands, which may be blocked away by modification of blocking conditions.

Additional information

Related products

Background

Background RHD3 (Protein Root Hair Defective 3) protein is probable GTP-binding protein involved in cell wall expansion. Required for appropriate root and root hair cells enlargement. May inhibit vacuole enlargement during root hair cell expansion. Plays a role in cell wall biosynthesis and actin organization. Seems to act independently from auxin and ethylene pathways. May regulate membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Localized in Golgi and ER membrane.
Alternative names: Fragile Fiber 4, Portein SEY1 homolog 1.

Product citations

Selected references Ueda et al. (2016). Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis. Plant Physiol. 2016 Feb;170(2):867-80. doi: 10.1104/pp.15.01172.

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