RHD3 | Protein Root Hair Defective 3 (N-terminal)
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Arabidopsis thaliana 7 days-old seedlings of wilde-type (1), rhd3-1 mutant (2), rhd3-2 mutant (3) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 15-20 % SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 100 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
Aterisks indicate non-specific bands, which may be blocked away by modification of blocking conditions.
Alternative names: Fragile Fiber 4, Portein SEY1 homolog 1.
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