Rnr3 | Ribonucleoside-diphosphate reductase large chain 2

AS09 574  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Saccharomyces cerevisiae

Rnr3 | Ribonucleoside-diphosphate reductase large chain 2 in the group Bacterial/Fungal Antibodies at Agrisera AB (Antibodies for research) (AS09 574)


381 €

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product information

Saccharomyces cerevisiae Rnr3 is catalyzing the biosynthesis of deoxyribonucelaotides. Alternative names: Ribonucleotide reductase large subunit 2, ribonucleotide reductase DNA damage-inducible regulatory subunit 2, ribonucleotide reductase R1 subunit 2


KLH-conjugated synthetic peptide derived from Saccharomyces cerevisiae Rnr3 protein sequence P21672

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 250 µg
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS10 847 | anti-Sml1 | Suppressor of Mec1 lethality

Secondary antibodies

Additional information

Recommended western blot protocol can be found here

application information
Recommended dilution 1 : 500-1 : 1000 (WB)
Expected | apparent MW

97.5 | 98 kDa

Confirmed reactivity Saccharomyces cerevisiae
Predicted reactivity

Saccharomyces cerevisiae

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Load per well was approx 3x10^6 cells (of a total extract).
Selected references Lafuente-Barquero et al. (2017). The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLOS Genetics, December 27, 2017,
Schmidt et al. (2017). Alterations in cellular metabolism triggered by URA7 or GLN3 inactivation cause imbalanced dNTP pools and increased mutagenesis. Proc Natl Acad Sci U S A. 2017 May 30;114(22):E4442-E4451. doi: 10.1073/pnas.1618714114.
Graf et al. (2017). Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle. Cell. 2017 Jun 29;170(1):72-85.e14. doi: 10.1016/j.cell.2017.06.006.
Williams et al. (2017). The role of RNase H2 in processing ribonucleotides incorporated during DNA replication. DNA Repair (Amst). 2017 Mar 6. pii: S1568-7864(16)30431-1. doi: 10.1016/j.dnarep.2017.02.016.

application example

western blot using anti-Rnr3 antibodies

10 μl total protein from 9.25 x 107 cells of Saccharomyces cerevisiae extracted with 20% TCA as described below were separated on 10% SDS-PAGE and blotted 1.5h (0.5 A) to a nitrocellulose membrane (Whatman PROTRAN BA 85, 0.45 μm). Blots were blocked with 5% non-fat dry milk in TBST for 1.5h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 200 overnight at 4C° with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, (AS09 602) diluted to 1:50 000 for 1h at RT with agitation. The blot was washed as above and developed for 3 min with SuperSignal West Pico Stable Solution (1856135 Thermo Scientific) and SuperSignal West Pico Luminol/Enhancer Solution (1856136 Thermo Scientific) mixed 1:1 rate according to the manufacturers instructions. Exposure time was 10 min.

Courtesy Dr. Andrei Chabes, Umeå University, Sweden