RPB2 | DNA-directed RNA polymerase II subunit 2

Product no: AS20 4376

AS20 4376   | Clonality: Polyclonal  |  Host: Chicken | Reactivity: Arabidopsis thaliana

Benefits of using this antibody

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana RPB2 UniProt: P38420-1, TAIR: AT4G21710


    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 135 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arachis duranensis, Brachypodium distachyon, Brassica campestris,Brassica oleracea, Brassica rapa, Capsicum annuum,Cucumis melo, Cucumis sativus, Hordeum vulgare, Homo sapiens, Lupinus angustifolius, Malus baccata, Nicotiana tabacum, Oryza sativa, Petunia hybrida, Phaseolus vulgaris, Populus alba, Raphanus sativus, Sorghum bicolor, Trifolium subterraneum, Triticum aestivum, Zea mays
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-RPB2 antibodies

    10-40 µg/well of total protein extracted from 7-d-old Arabidopsis thaliana seedlings (grown in continuous light at 22ºC) with 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25% Triton X-100, 2 mM PMSF and 1x protease inhibitor cocktail (Roche) and denatured with 1XLaemli buffer at 100°C for 5 min, were separated in duplicate on 7% SDS-PAGEs and blotted 1h 15 min to PVDF (0.2 um), using wet transfer. Blots were blocked with TBS-T/5% milk for 1h/RT with agitation. Blots were incubated in the primary antibodies at a dilution of 1:1000 (anti-NRPB2) ON/4°C in TBS-T/5% milk with agitation. The anti-NRPB2 antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (rabbit anti-chicken IgG horse radish peroxidase conjugated, AS10 1489) diluted to 1:1000 in for 1h/RT with agitation. The two blots were washed as above and developed for 5 min with chemiluminescent detection reagent, following manufacture's recommendations.  Exposure time was 10 seconds (ImageQuant 800, 3x3 bins; Amersham).

    Courtesy of Alberto Palacios-Abella from Dr. David Alabadi lab, CSIC-UPV, Spain

  • Background
  • Background: RPB2 (DNA-directed RNA polymerase II subunit 2) is a unique second-largest subunit of DNA-dependent RNA polymerase II.  Alternative names: DNA-directed RNA polymerase II 135 kDa polypeptide DNA-directed RNA polymerase II subunit RPB2 (EC:, RNA polymerase II subunit B2, Protein EMBRYO DEFECTIVE 1989
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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AS10 710 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. cinerea, C. reinhardtii, H. vulgare, S. lycopersicum, V. faba, P. tricornutum, P. patens, S. europaea, Z. mays | cellular [compartment marker] of nucleoplasm

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS07 225 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

319 €
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AS10 1489 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken IgG/Y (H&L)
218 €
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