RPL37 | Ribosomal protein L37 (cytoplasmic)

AS12 2115 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

RPL37 | Ribosomal protein L37 (cytoplasmic)

Product Information

Immunogen

Recombinant, full length RPL37 of Chlamydomonas reinhardtii, UniProt: A8IBG1, expressed in E.coli

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Liquid

Quantity 50 µl

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW 10,5 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Arabidopsis thaliana, Glycine max, Solanum lycopersicum, Oryza sativa, Ostreococcus lucimarinus, Pinus sp., Ricinus communis, Micromonas sp., Volvox carteri
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Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-RPL37 antibodies >

10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors) were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was washed 3 times for 15 min in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T containing 1% non-fat dry milk for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.

Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland

Reactant: Chlamydomonas reinhardtii (Green Alga)

Application: Western Blotting

Pudmed ID: 29053817

Journal: J Exp Bot

Figure Number: 3A,B,C,D

Published Date: 2018-03-14

First Author: Couso, I., Pérez-Pérez, M. E., et al.

Impact Factor: 6.088

Open Publication

Inhibition of autophagic flux by concanamycin A (ConcA) prevents the degradation of ribosomal proteins under nitrogen starvation or phosphate limitation. (A) Chlamydomonas cells growing exponentially in Tris-acetate phosphate medium (TAP) were washed twice with nitrogen-free medium (TAP-N) and grown under these conditions for 4, 8, and 24 h. Control cells were washed in TAP medium and grown in the presence of nitrogen. (B) Chlamydomonas cells growing in TAP medium were washed with a nitrogen-free medium and grown under these conditions for 16 h in the absence (–) or presence (+) of 0.1 µM ConcA. (C) Chlamydomonas cells growing exponentially in TAP medium were washed with a phosphate-free medium (TA) and grown under these conditions during 8, 24, and 48 h. Control cells were washed and resuspended in TAP medium. (D) Chlamydomonas cells growing in TAP medium were washed with a phosphate-free (TA) medium and grown under these conditions for 48 h. Before collecting samples, cells were treated for 24 h with 0.1 µM ConcA. For (A–D), 20 µg of total extracts were resolved by 12% (RPS6) or 15% (RPL37, ATG8, and FKBP12) SDS–PAGE followed by western blotting with anti-OLLAS, anti-RPL37, anti-ATG8, and anti-FKBP12 antibodies. Molecular mass markers (kDa) are indicated on the left.

Additional information

Additional information This product can be sold containing ProClin if requested

Related products

Plant and algal protein extraction buffer

Background

RPL37 (Ribosomal protein L37) belongs to the ribosomal protein L37e family (cytosolic large ribosomal subunit) and binds to the 23S rRNA.

Product citations

Selected references Ma et al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.
Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.
Couse et al. (2017). Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas. J Exp Bot. 2017 Oct 19. doi: 10.1093/jxb/erx372.
Couso et al. (2017). Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas. J Exp Bot. 2017 Oct 19. doi: 10.1093/jxb/erx372.
Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.