RPL37 | Ribosomal protein L37 (cytoplasmic)

Recombinant, full length RPL37 of Chlamydomonas reinhardtii, UniProt: A8IBG1, expressed in E.coli

10.5 kDa

 >

10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors)  were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk  for 1h at RT with agitation. The antibody solution was decanted and the blot was washed  3 times for 15 min  in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T containing 1% non-fat dry milk  for 1h at RT with agitation. The blot was washed as above  and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.
 

Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland

AS13 2650 | anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies
AS09 478| anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies
AS08 331| anti-L-30 | 50S ribosomal protein L30, rabbit antibodies
AS11 1738| anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies
AS15 2876 | anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies
AS14 2780 | anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies
AS14 2781 | anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodies
AS15 2875 | anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodies
AS12 2114 | anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodies
AS14 2779 | anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodies
AS10 719 | anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodies
AS08 309 | anti-S1 | anti-30S ribosomal protein S1, rabbit antibodies
AS12 2111 | anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodies
AS15 3068 | anti-S4 |   mitochondrial ribosomal small subunit protein S4, rabbit antibodies
AS15 3067 | anti-S10 |  mitochondrial ribosomal small subunit protein S10, rabbit antibodies
AS15 3069 | anti-L16   mitochondrial ribosomal large subunit protein L16, rabbit antibodies

Plant and algal protein extraction buffer

RPL37 (Ribosomal protein L37) belongs to the ribosomal protein L37e family (cytosolic large ribosomal subunit) and binds to the 23S rRNA.