RPL37 | Ribosomal protein L37 (cytoplasmic)

AS12 2115 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

Product Information

Immunogen

Recombinant, full length RPL37 of Chlamydomonas reinhardtii, UniProt: A8IBG1, expressed in E.coli

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW

10.5 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Arabidopsis thaliana, Glycine max, Solanum lycopersicum, Oryza sativa, Ostreococcus lucimarinus, Pinus sp., Ricinus communis, Micromonas sp., Volvox carteri
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Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-RPL37 antibodies >

10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors) were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was washed 3 times for 15 min in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T containing 1% non-fat dry milk for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.

Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland

Additional information

Additional information This product can be sold containing ProClin if requested.

Related products

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Background

RPL37 (Ribosomal protein L37) belongs to the ribosomal protein L37e family (cytosolic large ribosomal subunit) and binds to the 23S rRNA.

Product citations

Selected references Ma et al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.
Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.
Couse et al. (2017). Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas. J Exp Bot. 2017 Oct 19. doi: 10.1093/jxb/erx372.
Couso et al. (2017). Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas. J Exp Bot. 2017 Oct 19. doi: 10.1093/jxb/erx372.
Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.