RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (A.thaliana)

AS15 2845  |  Clonality: polyclonal  |  Host: rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana tabacum

RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (A.thaliana) in the group Antibodies for Plant/Algal  / Arabidopsis thaliana  at Agrisera AB (Antibodies for research) (AS15 2845)


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Product Information

Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RPN6 sequence, UniProt: Q9LP45-1, TAIR: AT1G29150
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution

1:1000 (WB)

Expected | apparent MW

46.7 kDa


Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamiana

Application examples

Application examples Western blot using anti-RPN6 antibodies

14 μg of total protein from Nicotiana benthamiana control and treated plants were extracted with Tris-EDTA buffer and denatured with Laemmli Sample Buffer 5x at 95 °C for 5 min were separated on 12% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 3% low-fat milk in TBS-T for 1h at room temperature (RT) with agitation. The blot was incubated with the primary antibody at a dilution of 1:3000 in TBS-T (overnight at +4 °C). The antibody solution was decanted, and the blot was rinsed briefly twice, and then washed 2 times for 5 min in TBS-T at RT with agitation. The blot was incubated using a matched secondary antibody (AS09 602, Rabbit anti-Goat IgG (H&L), HRP conjugated) diluted to 1:10 000 in blocking reagent for 1h at RT with agitation. The blot was washed as above and developed for 12 min with ECL reagent according to manufacture's recommendations.

Courtesy of Dr. Sandra Vitko, Department of Biology, Division of Molecular Biology, Croatia

Additional information

Related products

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AS15 2832 | anti-RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (other species), rabbit antibodies
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AS08 307A | anti-UBQ11 | Ubiquitin, affinity purified rabbit antibodies
AS10 307S | UBQ11 | Ubiquitin protein (positive control)



Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. RPN6 associates with an ATPase subunit of the 19S proteasome regulatory complex, AtS6A. 
Alternative names: 19S proteosome subunit 9, 26S proteasome non-ATPase regulatory subunit 11 homolog, AtRPN6.

Product citations

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Buy 2 items of this product for 218.00 €/items
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