RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (A.thaliana)
AS15 2845 | Clonality: polyclonal | Host: rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum
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Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
14 μg of total protein from Nicotiana benthamiana control and treated plants were extracted with Tris-EDTA buffer and denatured with Laemmli Sample Buffer 5x at 95 °C for 5 min were separated on 12% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 3% low-fat milk in TBS-T for 1h at room temperature (RT) with agitation. The blot was incubated with the primary antibody at a dilution of 1:3000 in TBS-T (overnight at +4 °C). The antibody solution was decanted, and the blot was rinsed briefly twice, and then washed 2 times for 5 min in TBS-T at RT with agitation. The blot was incubated using a matched secondary antibody (AS09 602, Rabbit anti-Goat IgG (H&L), HRP conjugated) diluted to 1:10 000 in blocking reagent for 1h at RT with agitation. The blot was washed as above and developed for 12 min with ECL reagent according to manufacture's recommendations.
Courtesy of Dr. Sandra Vitko, Department of Biology, Division of Molecular Biology, Croatia
Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. RPN6 associates with an ATPase subunit of the 19S proteasome regulatory complex, AtS6A.
Alternative names: 19S proteosome subunit 9, 26S proteasome non-ATPase regulatory subunit 11 homolog, AtRPN6.
Related products: RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (A.thaliana)
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