Rubisco quantitation kit (Western blot)
This set enables quantification of Rubisco using Western blot in samples from a range of various species including dicots, monocots, algae, cyanobacteria. Benefits of using this antibody set
|Data sheet||Product citations||Add review|
This set contains the following products:
AS03 037, RbcL | Rubisco large subunit, form I and form II (50 µl)
AS01 017S, Rubisco protein standard (100 µl)
AS08 300, Protein extraction buffer (2 x 2 ml)
AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated(2 x 10 µl)
AS16 ECL-N-10, AgriseraECL Bright (10 ml)
Species of your interest not listed? Contact us
2 µg of total protein from various plant extracts (1-5) extracted with Agriera Protein Extraction Buffer PEB (AS08 300) separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Markers MagicMarks (Invitrogen) (M) and Rubisco protein standard (AS01 017S) at 0.0625 pmol, 0.125 pmol, 0.25 pmol.
Following standard western blot procedure this image has been obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). The contour tool of the software is used to the area for quantitation and the values are background subtracted to give an adjusted volume in counts for each standard and sample.