RPS1 | 30S ribosomal protein S1
AS08 309 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. , Sylindropspermopsis raciborskii CS-505
DATA SHEET IN PDF| Datasheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Recombinant S1 protein of Synechocystis sp. strain PCC6803; one Flag-tag sequence (N-DYKDDDDK-C) was added to the C-terminus of the protein P73530
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
35 kDa
Reactivity
Confirmed reactivity
Synechocystis sp. strain PCC6803, Cylindrospermopsis raciborskii CS-505
Predicted reactivity
Algae, Arthrospira platensis, Cyanobacteria
Not reactive in
Escherichia coli
Application examples
Application examples
Application example
From left to right: Synechocystis, total cell extract; 10 μg/line (1); recombinant protein 2.5 ng (2); recombinant protein 5 ng (3); recombinant protein 10 ng (4); recombinant protein 20 ng (5)
Detection: chemiluminescence
Detection: chemiluminescence
Additional information
Background
Background
S1 ribosomal protein is involved in initiation of translation by recognition and binding of mRNAs through association with the 30S ribosomal subunit. S1 protein is localized in bacterial cytosol.
Product citations
Selected references
Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
Carmel at al. (2013). Structural model, physiology and regulation of Slr0006 in Synechocystis PCC 6803.Arch Microbiol. Sep 17.
Carmel at al. (2013). Structural model, physiology and regulation of Slr0006 in Synechocystis PCC 6803.Arch Microbiol. Sep 17.
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