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RPS1 | 30S ribosomal protein S1

AS08 309  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Synechocystis sp. , Sylindropspermopsis raciborskii CS-505

RPS1 | 30S ribosomal protein S1 in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS08 309)
RPS1 | 30S ribosomal protein S1



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Product Information

Immunogen

Recombinant S1 protein of Synechocystis sp. strain PCC6803; one Flag-tag sequence (N-DYKDDDDK-C) was added to the C-terminus of the protein P73530

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

35 kDa

Reactivity

Confirmed reactivity Synechocystis sp. strain PCC6803, Cylindrospermopsis raciborskii CS-505
Predicted reactivity Algae, Arthrospira platensis, Cyanobacteria
Species of your interest not listed? Contact us
Not reactive in Escherichia coli

Application examples

Application examples

Application example

AS08 309 western blot.jpg

 
From left to right: Synechocystis, total cell extract; 10 μg/line (1); recombinant protein 2.5 ng (2); recombinant protein 5 ng (3); recombinant protein 10 ng (4); recombinant protein 20 ng (5)
Detection: chemiluminescence

Reactant: Synechocystis

Application: Western Blotting

Pudmed ID: 29985458

Journal: Sci Rep

Figure Number: 2A

Published Date: 2018-07-09

First Author: Koskinen, S., Hakkila, K., et al.

Impact Factor: 4.13

Open Publication

Ribosomal proteins, translation activity and total protein contents in the ?sigBCDE and control (CS) strains in standard growth conditions. (A) Total proteins were isolated, separated by SDS-PAGE and then the Rps1 protein of the 30S ribosomal subunit and the Rpl1 protein of the 50S ribosomal subunit were detected with western blotting. As a loading control the amount of the ? subunit of ATPase was detected as well. Original Western blots are shown in Supplementary Fig. S1. (B) Cells were labelled with radioactive 35S-methionine for 10 or 30?min, as indicated, in standard growth conditions, and total proteins were isolated after addition of excess of cold methionine. Proteins were separated by SDS-PAGE, transferred to the membrane and visualized by autoradiography. (C) Total proteins were isolated from the same amount of cells and the protein content was detected with a BioRad DC protein assay kit. Three independent biological replicates were measured, Student’s t-test P?=?0.905.


Reactant: Synechocystis

Application: Western Blotting

Pudmed ID: 29985458

Journal: Sci Rep

Figure Number: 3B

Published Date: 2018-07-09

First Author: Koskinen, S., Hakkila, K., et al.

Impact Factor: 4.13

Open Publication

Ribosome profiles of the control (CS) and ?sigBCDE strains. The ribosomes of the CS (A,B) and ?sigBCDE (C,D) strains were fractionated with a 5–35% sucrose density gradient centrifugation, nineteen fractions were collected and either RNA or ribosomal proteins Rps1 and Rpl1 were detected from each fraction. RNA was isolated from the 300??L sample of fraction, dissolved in 20??L of water, and 15??L of isolated RNA from each fraction of CS (A) or ?sigBCDE (C) was loaded on a 1.2% agarose gel and stained with ethidium bromide. The DNA molecular marker was included to demonstrate separation of fragments. For western blots, a 24??L sample of a fraction was solubilized and proteins were separated on SDS-PAGE and Rps1 and Rpl1 proteins were detected by specific antibodies by western blotting in the control (B) and ?sigBCDE (D) strains.


Reactant: Synechocystis

Application: Western Blotting

Pudmed ID: 29985458

Journal: Sci Rep

Figure Number: 3D

Published Date: 2018-07-09

First Author: Koskinen, S., Hakkila, K., et al.

Impact Factor: 4.13

Open Publication

Ribosome profiles of the control (CS) and ?sigBCDE strains. The ribosomes of the CS (A,B) and ?sigBCDE (C,D) strains were fractionated with a 5–35% sucrose density gradient centrifugation, nineteen fractions were collected and either RNA or ribosomal proteins Rps1 and Rpl1 were detected from each fraction. RNA was isolated from the 300??L sample of fraction, dissolved in 20??L of water, and 15??L of isolated RNA from each fraction of CS (A) or ?sigBCDE (C) was loaded on a 1.2% agarose gel and stained with ethidium bromide. The DNA molecular marker was included to demonstrate separation of fragments. For western blots, a 24??L sample of a fraction was solubilized and proteins were separated on SDS-PAGE and Rps1 and Rpl1 proteins were detected by specific antibodies by western blotting in the control (B) and ?sigBCDE (D) strains.

Additional information

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Background

Background

S1 ribosomal protein is involved in initiation of translation by recognition and binding of mRNAs through association with the 30S ribosomal subunit. S1 protein is localized in bacterial cytosol.

Product citations

Selected references Carrieri et al. (2021) Overexpression of NblA decreases phycobilisome content and enhances photosynthetic growth of the cyanobacterium Synechococcus elongatus PCC 7942,Algal Research,Volume 60, 2021, 102510, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2021.102510.
Zav?el et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.
Koskinen et al. (2018). Inactivation of group 2 ? factors upregulates production of transcription and translation machineries in the cyanobacterium Synechocystis sp. PCC 6803. Sci Rep. 2018 Jul 9;8(1):10305. doi: 10.1038/s41598-018-28736-9.
Kurkela et al. (2017). Acclimation to High CO2 Requires the ? Subunit of the RNA Polymerase in Synechocystis. Plant Physiol. 2017 May;174(1):172-184. doi: 10.1104/pp.16.01953. Epub 2017 Mar 28.
Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
Carmel at al. (2013). Structural model, physiology and regulation of Slr0006 in Synechocystis PCC 6803.Arch Microbiol. Sep 17.

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