RPS1 | 30S ribosomal protein S1

Product no: AS08 309

AS08 309  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Synechocystis sp. , Sylindropspermopsis raciborskii CS-505

Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
302
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Recombinant S1 protein of Synechocystis sp. strain PCC6803; one Flag-tag sequence (N-DYKDDDDK-C) was added to the C-terminus of the protein P73530

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    35 kDa

  • Reactivity
  • Confirmed reactivity: Synechocystis sp. strain PCC6803, Cylindrospermopsis raciborskii CS-505
    Predicted reactivity: Algae, Arthrospira platensis, Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: Escherichia coli
  • Application Examples
  • Application example

    AS08 309 western blot.jpg

     
    From left to right: Synechocystis, total cell extract; 10 μg/line (1); recombinant protein 2.5 ng (2); recombinant protein 5 ng (3); recombinant protein 10 ng (4); recombinant protein 20 ng (5)
    Detection: chemiluminescence

    Application examples:

    Reactant: Synechocystis

    Application: Western Blotting

    Pudmed ID: 29985458

    Journal: Sci Rep

    Figure Number: 2A

    Published Date: 2018-07-09

    First Author: Koskinen, S., Hakkila, K., et al.

    Impact Factor: 4.13

    Open Publication

    Ribosomal proteins, translation activity and total protein contents in the ?sigBCDE and control (CS) strains in standard growth conditions. (A) Total proteins were isolated, separated by SDS-PAGE and then the Rps1 protein of the 30S ribosomal subunit and the Rpl1 protein of the 50S ribosomal subunit were detected with western blotting. As a loading control the amount of the ? subunit of ATPase was detected as well. Original Western blots are shown in Supplementary Fig. S1. (B) Cells were labelled with radioactive 35S-methionine for 10 or 30?min, as indicated, in standard growth conditions, and total proteins were isolated after addition of excess of cold methionine. Proteins were separated by SDS-PAGE, transferred to the membrane and visualized by autoradiography. (C) Total proteins were isolated from the same amount of cells and the protein content was detected with a BioRad DC protein assay kit. Three independent biological replicates were measured, Student’s t-test P?=?0.905.


    Reactant: Synechocystis

    Application: Western Blotting

    Pudmed ID: 29985458

    Journal: Sci Rep

    Figure Number: 3B

    Published Date: 2018-07-09

    First Author: Koskinen, S., Hakkila, K., et al.

    Impact Factor: 4.13

    Open Publication

    Ribosome profiles of the control (CS) and ?sigBCDE strains. The ribosomes of the CS (A,B) and ?sigBCDE (C,D) strains were fractionated with a 5–35% sucrose density gradient centrifugation, nineteen fractions were collected and either RNA or ribosomal proteins Rps1 and Rpl1 were detected from each fraction. RNA was isolated from the 300??L sample of fraction, dissolved in 20??L of water, and 15??L of isolated RNA from each fraction of CS (A) or ?sigBCDE (C) was loaded on a 1.2% agarose gel and stained with ethidium bromide. The DNA molecular marker was included to demonstrate separation of fragments. For western blots, a 24??L sample of a fraction was solubilized and proteins were separated on SDS-PAGE and Rps1 and Rpl1 proteins were detected by specific antibodies by western blotting in the control (B) and ?sigBCDE (D) strains.


    Reactant: Synechocystis

    Application: Western Blotting

    Pudmed ID: 29985458

    Journal: Sci Rep

    Figure Number: 3D

    Published Date: 2018-07-09

    First Author: Koskinen, S., Hakkila, K., et al.

    Impact Factor: 4.13

    Open Publication

    Ribosome profiles of the control (CS) and ?sigBCDE strains. The ribosomes of the CS (A,B) and ?sigBCDE (C,D) strains were fractionated with a 5–35% sucrose density gradient centrifugation, nineteen fractions were collected and either RNA or ribosomal proteins Rps1 and Rpl1 were detected from each fraction. RNA was isolated from the 300??L sample of fraction, dissolved in 20??L of water, and 15??L of isolated RNA from each fraction of CS (A) or ?sigBCDE (C) was loaded on a 1.2% agarose gel and stained with ethidium bromide. The DNA molecular marker was included to demonstrate separation of fragments. For western blots, a 24??L sample of a fraction was solubilized and proteins were separated on SDS-PAGE and Rps1 and Rpl1 proteins were detected by specific antibodies by western blotting in the control (B) and ?sigBCDE (D) strains.

  • Background
  • Background:

    S1 ribosomal protein is involved in initiation of translation by recognition and binding of mRNAs through association with the 30S ribosomal subunit. S1 protein is localized in bacterial cytosol.

  • Product Citations
  • Selected references: Carrieri et al. (2021) Overexpression of NblA decreases phycobilisome content and enhances photosynthetic growth of the cyanobacterium Synechococcus elongatus PCC 7942,Algal Research,Volume 60, 2021, 102510, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2021.102510.
    Zav?el et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.
    Koskinen et al. (2018). Inactivation of group 2 ? factors upregulates production of transcription and translation machineries in the cyanobacterium Synechocystis sp. PCC 6803. Sci Rep. 2018 Jul 9;8(1):10305. doi: 10.1038/s41598-018-28736-9.
    Kurkela et al. (2017). Acclimation to High CO2 Requires the ? Subunit of the RNA Polymerase in Synechocystis. Plant Physiol. 2017 May;174(1):172-184. doi: 10.1104/pp.16.01953. Epub 2017 Mar 28.
    Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
    Carmel at al. (2013). Structural model, physiology and regulation of Slr0006 in Synechocystis PCC 6803.Arch Microbiol. Sep 17.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info

AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206 €
Add to cart
Info
Close