Choose currency:
ANTIBODY SHOP
Name:
Phone:
E-mail:
Address:
Send message

SARD1 | Protein SAR Deficient 1

AS21 4599  | Clonality:  Polyclonal |  Host:  Rabbit  | Reactivity: Arabidopsis Thaliana, Arabidopsis arenosa, Arabidopsis suecica, Arabidopsis lyrata subsp. lyrata, Camelina sativa, Sinapis alba, Brassica oleracea, Brassica rapa, Raphanus sativus, Capsella rubella

SARD1 | Protein SAR Deficient 1 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS21 4599)

Data sheet Protocols Add review
immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana SARD1 protein sequence, UniProt: Q9C9T2, TAIR: At1g73805
Host:

Rabbit
Clonality:

Polyclonal
Purity: Affinity purified serum, in PBS pH 7.4
Format:

Lyophilized
Quantity: 50 µg
Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
Expected | apparent MW: 50.7 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Arabidopsis arenosa, Arabidopsis suecica, Arabidopsis lyrata subsp. lyrata, Camelina sativa, Sinapis alba, Brassica oleracea, Brassica rapa, Raphanus sativus, Capsella rubella


Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer): application example



µg of total protein extracted freshly from    with write exact buffer components ..... and denatured with....at °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

Courtesy of
background: Background info.

Related products: SARD1 | Protein SAR Deficient 1

Agrisera ECL kit (Bright/SuperBright)
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only...
From 26 €
Info Add to cart
Goat anti-Rabbit IgG (H&L), HRP conjugated
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
Info Add to cart