S protein of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)

AS20 4386 | Clonality: Recombinant Monoclonal | Host: Mouse | Reactivity: S protein Human SARS-CoV-2/ 2019-nCoV

Product Information

Immunogen Recombinant Human Novel Coronavirus Spike glycoprotein (S) (16-685aa), UniProt: P0DTC2 Antigen sequence has highest homology to Spike protein S1.

Host

Mouse

Clonality Monoclonal recombinant

Subclass/isotype Mouse scFv fusion with human IgG1 Fc

Purity Immunoglobulin purified on Protein A in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.

Format Liquid

Quantity 100 µl

Storage Store at -20°C or -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Colloidlal Gold Immunochromatography, ELISA (ELISA)

Recommended dilution 1: 500 - 1: 40 000 (Colloidal Gold Immunochromatography), 1: 10 000 - 1: 50 000 (ELISA)

Expected | apparent MW 141 kDa

Reactivity

Confirmed reactivity Human Novel SARS-CoV-2/ 2019-nCoV S protein

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Binding activity of SARS-CoV-2-S antibodies

Binding Activity of SARS-CoV-2-S Antibody measured by its binding ability in a functional ELISA. Wells were coated with Recombinant Human Novel Coronavirus Spike glycoprotein(S) (AS20 4389) at 2 μg/ml. EC₅₀ of SARS-CoV-2-S antibodies is 36.79 - 48.87 ng/ml.

Additional information

Recombinant anti-SARS-CoV-2 spike Mouse ScFv is expressed in 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. Therefore, anti-human IgG1 Fcγ (gamma chain), secondary antibody has to be used: AS20 4390

Background

Background SARS-CoV-2/ 2019-nCoV S protein of Novel Coronavirus (human) its role is to attache the virion to the cell membrane by interacting with host receptor (ACE2) and initiating the infection.

Alternative names: S, S1, S1-RBD, Spike glycoprotein.