S protein of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
AS20 4386 | Clonality: Recombinant Monoclonal | Host: Mouse | Reactivity: S protein Human SARS-CoV-2/ 2019-nCoV

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Product Information
Immunogen
Recombinant Human Novel Coronavirus Spike glycoprotein (S) (16-685aa), UniProt: P0DTC2 Antigen sequence has highest homology to Spike protein S1.
Host
Mouse
Clonality
Monoclonal recombinant
Subclass/isotype
Mouse scFv fusion with human IgG1 Fc
Purity
Immunoglobulin purified on Protein A in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
Format
Liquid
Quantity
100 ĩl
Storage
Store at -20°C or -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Colloidlal Gold Immunochromatography, ELISA (ELISA)
Recommended dilution
1: 500 - 1: 40 000 (Colloidal Gold Immunochromatography), 1: 10 000 - 1: 50 000 (ELISA)
Expected | apparent MW
141 kDa
Reactivity
Confirmed reactivity
Human Novel SARS-CoV-2/ 2019-nCoV S protein
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example

Binding Activity of SARS-CoV-2-S Antibody measured by its binding ability in a functional ELISA. Wells were coated with Recombinant Human Novel Coronavirus Spike glycoprotein(S) (AS20 4389) at 2 μg/ml. EC50 of SARS-CoV-2-S antibodies is 36.79 - 48.87 ng/ml.
Additional information
Recombinant anti-SARS-CoV-2 spike Mouse ScFv is expressed in 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. Therefore, anti-human IgG1 Fcγ (gamma chain), secondary antibody has to be used: AS20 4390
Background
Background
SARS-CoV-2/ 2019-nCoV S protein of Novel Coronavirus (human) its role is to attache the virion to the cell membrane by interacting with host receptor (ACE2) and initiating the infection.
Alternative names: S, S1, S1-RBD, Spike glycoprotein.
Alternative names: S, S1, S1-RBD, Spike glycoprotein.
Product citations
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