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Anti-SE | Serrate RNA effector molecule (chicken antibody)
Product no: AS15 2836
AS15 2836 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana
329
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence UniProt: Q9ZVD0,TAIR: At2g27100
Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB) Expected | apparent MW: 81 | 80 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Malus domestica, Nicotiana benthamina, Nicotiana tabacum, Saccharum hybrid cultivar NCo 376, Zea mays
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example 
30 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 10 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween for 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS10 1489 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with chemiluminescent detection reagent, according to manufacturer's instructions. Exposure time was 10 min.
Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland - Additional Information
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Additional information (application): Over night incubation with anti-serrate antibodies is not recommended as it can contribute to increased background signal - Background
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Background: Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).
- Protocols
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