SE | Serrate RNA effector molecule (chicken antibody)

Name: SE | Serrate RNA effector molecule (chicken antibody)
SKU: AS15 2836
Price: 302 EUR
Availability: InStock

AS15 2836 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana

SE | Serrate RNA effector molecule (chicken antibody) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / plant RNA at Agrisera AB (Antibodies for research) (AS15 2836)

SE | Serrate RNA effector molecule (chicken antibody)

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence UniProt: Q9ZVD0,TAIR: At2g27100

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

81 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Malus domestica, Nicotiana benthamina, Nicotiana tabacum, Saccharum hybrid cultivar NCo 376, Zea mays
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using chicken anti-serrate antibodies

30 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 10 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween for 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS10 1489 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with chemiluminescent detection reagent, according to manufacturer's instructions. Exposure time was 10 min.

Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland

Additional information

Over night incubation with anti-serrate antibodies is not recommended as it can contribute to increased background signal

Background

Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).

Product citations

immunogen:	KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence UniProt: Q9ZVD0,TAIR: At2g27100
Host:	Chicken
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 2000 (WB)
calculated \| apparent molecular mass [kDa]:	81 \| 80 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Malus domestica, Nicotiana benthamina, Nicotiana tabacum, Saccharum hybrid cultivar NCo 376, Zea mays Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 30 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 10 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween for 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS10 1489 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with chemiluminescent detection reagent, according to manufacturer's instructions. Exposure time was 10 min. Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland
additional information (application):	Over night incubation with anti-serrate antibodies is not recommended as it can contribute to increased background signal
background:	Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).