SE | Serrate RNA effector molecule (rabbit antibody)
AS09 532A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana benthamina, Nicotiana tabacum
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81 | 80 kDa
25-30 µg of total protein from Arabidopsis thaliana rosette leaves was extracted with extraction buffer containing: 100 mM Tris HCl pH 7.5, 10 % sucrose, 5 mM EDTA, 5 mM EGTA, 300 mM NaCl, 0.75 % Triton X100, 0.15 % SDS, 1 mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) or 7.5 % nuclear fraction obtained according to the protocol from Raczyńska et al. 2014, were separated on 10 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 2.5 % milk in PBS/T for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 250 in PBS-T for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit HRP conjugated, AS09 602, Agrisera) in 1: 5000 dlution for 1 h at RT with agitation. The blot was washed as above and developed for 5 minutes with ECL according to manufacturer's instructions. Exposure time was 600 seconds.
Courtesy of M.Sc. Mateusz Bajczyk, Department of Gene Expression, Adam Mickiewicz University, Poland
Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).
Wang et al. (2019). The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis. Plant Cell. 2019 Feb;31(2):486-501. doi: 10.1105/tpc.18.00556.
Kryovrysanaki et al. (2018). SERRATE, a miRNA biogenesis factor, affects viroid infection in Nicotiana benthamiana and Nicotiana tabacum. Virology. 2018 Dec 29;528:164-175. doi: 10.1016/j.virol.2018.12.011.
Ma et al. (2018). Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression. Dev Cell. 2018 Jun 18;45(6):769-784.e6. doi: 10.1016/j.devcel.2018.05.023.
Wang et al. (2018). SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. Nature. 2018 May;557(7706):516-521. doi: 10.1038/s41586-018-0135-x. Epub 2018 May 16.
Li et al. (2016). Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016 Nov 21;12(11):e1006422. doi: 10.1371/journal.pgen.1006422. eCollection 2016.
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