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SE | Serrate RNA effector molecule (rabbit antibody)

AS09 532A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamina, Nicotiana tabacum

SE | Serrate RNA effector molecule (rabbit antibody) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS09 532A)

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Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence Q9ZVD0, At2g27100

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), RIP (RNA Immunoprecipitation (IP) assay), Western blot (WB)
Recommended dilution 1 : 500 (IL), 1 : 1000 (WB)
Expected | apparent MW

81 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamina, Nicotiana tabacum
Predicted reactivity Malus domestica, Saccharum hybrid cultivar NCo 376, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
Application example

western blot using anti-serrate antibodies

25-30 µg of total protein from Arabidopsis thaliana rosette leaves was extracted with extraction buffer containing: 100 mM Tris HCl pH 7.5, 10 % sucrose, 5 mM EDTA, 5 mM EGTA, 300 mM NaCl, 0.75 % Triton X100, 0.15 % SDS, 1 mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) or 7.5 % nuclear fraction obtained according to the protocol from Raczyńska et al. 2014, were separated on 10 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 2.5 % milk in PBS/T for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 250 in PBS-T for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit HRP conjugated, AS09 602, Agrisera) in 1: 5000 dlution for 1 h at RT with agitation. The blot was washed as above and developed for 5 minutes with ECL according to manufacturer's instructions. Exposure time was 600 seconds.

Courtesy of M.Sc. Mateusz Bajczyk, Department of Gene Expression, Adam Mickiewicz University, Poland

Additional information

Suggested blotting conditions: 8% gel, tank blotting, 200 mA/ 1h to nitrocellulose membrane.

Related products

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AS15 2836 | Anti-Serrate RNA effector molecule, chicken antibodies

Collection of antibodies to micro RNA

Background

Background

Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).

Product citations

Selected references Wang et al. (2019). The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis. Plant Cell. 2019 Feb;31(2):486-501. doi: 10.1105/tpc.18.00556. 
Kryovrysanaki et al. (2018). SERRATE, a miRNA biogenesis factor, affects viroid infection in Nicotiana benthamiana and Nicotiana tabacum. Virology. 2018 Dec 29;528:164-175. doi: 10.1016/j.virol.2018.12.011.
Ma et al. (2018). Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression. Dev Cell. 2018 Jun 18;45(6):769-784.e6. doi: 10.1016/j.devcel.2018.05.023.
Wang et al. (2018). SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. Nature. 2018 May;557(7706):516-521. doi: 10.1038/s41586-018-0135-x. Epub 2018 May 16.
Li et al. (2016). Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016 Nov 21;12(11):e1006422. doi: 10.1371/journal.pgen.1006422. eCollection 2016.

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