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Anti-Cat3 | Catalase 3
AS23 4987 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana catalase 3 protein sequence, UniProt: Uniprot: Q42547-1TAIR: AT1G20620 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 56.7 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica napus
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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20 µl/well of total protein extracted freshly from Arabidopsis thaliana with extraction buffer containing: protease inhibitor, glycerol, sodium phosphate buffer, EDTQ, NaCl, SDS, Mg132, NP-40, sodium deoxycholate and denatured with heat-block at 80°C for 5 min. Col-0 Arabidopsis thaliana (1) and cat2/3 double mutant Arabidopsis thaliana (2) were separated on 10% SDS-Page and blotted 1 h to nitrocellulose (pore size of 0.45 µm), using semi-dry transfer. Blot was blocked with 5% skim milk (2.5 g skim milk powder + 50 mL TBS (1X)) 65 rpm / 1 hr /RT. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 4 hr/RT with agitation in 1% skim-milk + TBS-T with agitation in the dark. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in TBS for 1 h/RT with agitation. The antibody solution was decanted, and the blot was rinsed using 1X TBS-T at RT with agitation for 3 times, each taking 15 min, total of 45 min. The blot was washed as above and developed for 1m with chemiluminescent detection reagent of femtogram sensitivity. Exposure time was 6 minutes. - Background
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Background: CAT3 (Catalase 3) is a protein in Arabidopsis thaliana that breaks down hydrogen peroxide to protect cells from oxidative damage. It helps maintain redox balance during metabolism and stress. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 501 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.madagascariensis, B. distachyon, B.oleracea, Cirtus sp., Fragaria x ananassa, H.vulgare, L. sativus, L. albus, M. perniciosa, N.bentamina, N.tabacum, O.sativa, P. kurroa, P.sativum, P. zeylandica, P.tomentosa, R.sativus, S. italica, S.lycopersicum, S.oleracea, T.aestivum, Z.mays, V. vinifera
Benefits of using this antibody
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