GOX | Glycolate oxidase 1,2,3
AS14 2772 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum, Oryza sativa
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15 µg of total protein from Arabidopsis thaliana leaves extracted with 50 mM Tris-HCl (pH 7.8), 0.2% Tritin X-100, 0.1 mM EDTA and proteases inhbitors, were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with TBST containing 3% milk powder for 1h at room temperature (RT) and overnight at 4º with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was from 30 seconds to 2 minutes.
Courtesy of Dr. Luisa M. Sandalio-González, CSIC, Spain
Alternative name: Peroxisomal (S)-2-hydroxy-acid oxidase GLO.
Umnajkitikorn et al. (2020). Silencing of OsCV (chloroplast vesiculation) maintained photorespiration and N assimilation in rice plants grown under elevated CO2. Plant Cell Environ . 2020 Apr;43(4):920-933. doi: 10.1111/pce.13723.
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