GOX | Glycolate oxidase 1,2,3

AS14 2772  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

GOX | Glycolate oxidase 1,2,3 in the group Plant/Algal Antibodies / Environmental Stress / Pathogen attack at Agrisera AB (Antibodies for research) (AS14 2772)


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product information
Background Glycolate oxidase (GOX) is an enzyme which  modulates reactive oxygen species-mediated signal transduction during nonhost resistance. There are three GOX isoforms in Arabidopsis thaliana: GOX1,2,3. Alternative name: Peroxisomal (S)-2-hydroxy-acid oxidase GLO.

KLH-conjugated peptide derived from Arabidopsis thaliana GOX1 UniProt: Q9LRR9,TAIR: AT3G14420,  GOX2 UniProt: Q9LRS0, TAIR: AT3G14415, GOX3 UniProt: Q24JJ8, TAIR:

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to proteins involved in pathogen attack

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW
Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum sp. Zea mays, Vitis vinifera
Not reactive in

Gracilaria lemaneiformis

Additional information
Selected references

to be added when available, antibody released in February 2015

application example

western blot using anti-GOX antibodies 

15 µg of total protein from Arabidopsis thaliana leaves extracted with 50 mM Tris-HCl (pH 7.8), 0.2% Tritin X-100, 0.1 mM EDTA and proteases inhbitors, were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with TBST containing 3% milk powder for 1h at room temperature (RT) and overnight at 4º with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions (Amersham). Exposure time was from 30 seconds to 2 minutes.

Courtesy of Dr. Luisa M. Sandalio-González, CSIC, Spain

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