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GOX | Glycolate oxidase 1,2,3

AS14 2772  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Oryza sativa

GOX | Glycolate oxidase 1,2,3 in the group Antibodies for Plant/Algal  / Environmental Stress / Pathogen attack at Agrisera AB (Antibodies for research) (AS14 2772)

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Product Information

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana GOX1 UniProt: Q9LRR9,TAIR: AT3G14420,  GOX2 UniProt: Q9LRS0, TAIR: AT3G14415, GOX3 UniProt: Q24JJ8, TAIR:

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Oryza sativa
Predicted reactivity Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum,  Phaseolus vulgaris, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum sp. Zea mays, Vitis vinifera

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Not reactive in

Gracilaria lemaneiformis

Application examples

Application examples

application example

western blot using anti-GOX antibodies 

15 µg of total protein from Arabidopsis thaliana leaves extracted with 50 mM Tris-HCl (pH 7.8), 0.2% Tritin X-100, 0.1 mM EDTA and proteases inhbitors, were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with TBST containing 3% milk powder for 1h at room temperature (RT) and overnight at 4º with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was from 30 seconds to 2 minutes.

Courtesy of Dr. Luisa M. Sandalio-González, CSIC, Spain

Additional information

Related products

Background

Background Glycolate oxidase (GOX) is an enzyme which  modulates reactive oxygen species-mediated signal transduction during nonhost resistance. There are three GOX isoforms in Arabidopsis thaliana: GOX1,2,3.

Alternative name: Peroxisomal (S)-2-hydroxy-acid oxidase GLO.

Product citations

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