SPSA1 | Sucrose phosphate synthase isoform A1

Product no: AS15 2995

AS15 2995  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPSA1 sequence, Uniprot: Q56X42, TAIR: AT5G20280

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Expected | apparent MW: 117,3 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arabidopsis alpina, Brassica napus, Eutremas alsugineum, Glycine max, Glycine soja
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-SPSA1 antibody

    Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 12 µg of proteins were loaded into each lane and separated on 8% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 1 000) overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with Agrisera ECL Bright according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.
  • Background
  • Background:

    SPSA1 catalyzes the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose-6-phosphate. It plays a role in regulation of carbon partitioning in the leaves of plants. Furthermore, it is important for sucrose availability which is essential for plant growth and fiber elongation. SPSA1 is also necessary for nectar secretion.

    Alternative names: Sucrose-phosphate synthase 1, SPS1,
    Sucrose-phosphate synthase 1F, SPS1F, Sucrose-phosphate synthase 5.1, SPS5.1, UDP-glucose-fructose-phosphate glucosyltransferase

     

  • Product Citations
  • Selected references: 38221900
  • Protocols


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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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