SPSA1 | Sucrose phosphate synthase isoform A1

Name: SPSA1 | Sucrose phosphate synthase isoform A1
SKU: AS15 2995
Price: 302 EUR
Availability: InStock

AS15 2995 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SPSA1 | Sucrose phosphate synthase isoform A1 in the group Antibodies Plant/Algal / Photosynthesis / Sucrose metabolism at Agrisera AB (Antibodies for research) (AS15 2995)

SPSA1 | Sucrose phosphate synthase isoform A1

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPSA1 sequence, Uniprot: Q56X42, TAIR: AT5G20280

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Expected | apparent MW 117,3 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Arabidopsis alpina, Brassica napus, Eutremas alsugineum, Glycine max, Glycine soja
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

Western blot using anti-SPSA1 antibody

Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 12 µg of proteins were loaded into each lane and separated on 8% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 1 000) overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with Agrisera ECL Bright according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.

Additional information

Background

SPSA1 catalyzes the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose-6-phosphate. It plays a role in regulation of carbon partitioning in the leaves of plants. Furthermore, it is important for sucrose availability which is essential for plant growth and fiber elongation. SPSA1 is also necessary for nectar secretion.

Alternative names: Sucrose-phosphate synthase 1, SPS1, Sucrose-phosphate synthase 1F, SPS1F, Sucrose-phosphate synthase 5.1, SPS5.1, UDP-glucose-fructose-phosphate glucosyltransferase

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPSA1 sequence, Uniprot: Q56X42, TAIR: AT5G20280
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
calculated \| apparent molecular mass [kDa]:	117,3 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Arabidopsis alpina, Brassica napus, Eutremas alsugineum, Glycine max, Glycine soja Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 12 µg of proteins were loaded into each lane and separated on 8% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 1 000) overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with Agrisera ECL Bright according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.
background:	SPSA1 catalyzes the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose-6-phosphate. It plays a role in regulation of carbon partitioning in the leaves of plants. Furthermore, it is important for sucrose availability which is essential for plant growth and fiber elongation. SPSA1 is also necessary for nectar secretion. Alternative names: Sucrose-phosphate synthase 1, SPS1, Sucrose-phosphate synthase 1F, SPS1F, Sucrose-phosphate synthase 5.1, SPS5.1, UDP-glucose-fructose-phosphate glucosyltransferase