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SPS | Sucrose phosphate synthase (maize)

AS06 185   |  Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Alfalfa, Z.mays, S.lycopersicum   | cellular [compartment marker] of cytoplasm

SPS | Sucrose phosphate synthase (maize) in the group Antibodies for Plant/Algal  / Carbohydrates at Agrisera AB (Antibodies for research) (AS06 185)

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Product Information

Immunogen

Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 1500 (IL), 1 : 2000 (WB)
Expected | apparent MW

120 | ~130 for Zea mays

Reactivity

Confirmed reactivity Alfalfa, Solanum lycopersicum, Zea mays
Predicted reactivity Oryza sativa, Saccharum officinarum, Triticum aestivum
Not reactive in Hordeum vulgare

Application examples

Application examples

Application example

10 µg of total leaf protein from Arabidopsis thaliana (1,2), Zea mays (3) and Hordeum vulgare (4), extracted with Agrisera Protein Extraction Buffer, PEB (AS08 300) as well as 10 µg cytosolic protein from Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescence detection reagent, using a Fuji LAS-3000 CCD (90s, high sensitivity).

Additional information

Additional information

Total IgG concentration is 3 µg/µl

Related products

Related products
AS03 035A | Anti-SPS | Sucrose phosphate synthase, global, rabbit antibodies
AS03 035-HRP | Anti-SPS | Sucrose phosphate synthase, global (40 µg, HRP-conjugated), rabbit antibodies
AS03 035-ALP | Anti-SPS | Sucrose phosphate synthase, global (40 µg, ALP-conjugated), rabbit antibodies
AS15 2995 | Anti-SPSA1 | Sucrose phosphate synthase isoform A1, rabbit antibodies
AS15 2996 | Anti-SPSA2 | Sucrose phosphate synthase isoform A1, rabbit antibodies
AS15 2997 | Anti-SPSC | Sucrose phosphate synthase isoform C, rabbit antibodies

Plant and algal protein extraction buffer

Background

Background

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Product citations

Selected references Padhi et al. (2019). Distinct nodule and leaf functions of two different sucrose phosphate synthases in alfalfa. Planta (2019). https://doi.org/10.1007/s00425-019-03261-9.
Kaur et al. (2019). Comparison of alfalfa plants overexpressing glutamine synthetase with those overexpressing sucrose phosphate synthase demonstrates a signaling mechanism integrating carbon and nitrogen metabolism between the leaves and nodules. Plant Direct, Volume3, Issue1, Jan 2019, e00115.
Seger et al. (2014). Impact of concurrent overexpression of cytosolic glutamine synthetase (GS 1 ) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco. Planta. 2014 Sep 12.
Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J Plant Growth Regul. DOI 10.1007/s00344-014-9441-1. (immunolocalization)

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