SPS | Sucrose phosphate synthase (maize)

Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

120 | ~130 for Zea mays

Application example

10 µg of total leaf protein from Arabidopsis thaliana (1,2), Zea mays (3) and Hordeum vulgare (4), extracted with Agrisera Protein Extraction Buffer, PEB (AS08 300) as well as 10 µg cytosolic protein from Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescence detection reagent, using a Fuji LAS-3000 CCD (90s, high sensitivity).

Total IgG concentration is 3 µg/µl

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.